C57BL/6 mice were purchased from the National Cancer Institute (Frederick, MD) for use as wild-type mice and were housed in the animal facilities at the University of Maryland, College Park prior to use in experiments. CCR2−/− mice (Strain #: 004999), Nr4a1−/− mice (Strain #: 006187), and CSF2−/− mice (Strain #: 026812), all in the C57BL/6 background were purchased from The Jackson Laboratory (Bar Harbor, ME, USA), and bred in-house. All mice were maintained in specific-pathogen-free conditions in individually ventilated cages with a standard 12 h light/dark cycle. For experiments, age- and sex-matched, male or female 6- to 8-week-old mice were used. The study protocol was approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Maryland, College Park. All animal studies were conducted in accordance with the National Institutes of Health guidelines.
Csf2 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
CSF2−/− mice are genetically modified mice that lack the gene encoding for granulocyte-macrophage colony-stimulating factor (GM-CSF). These mice are useful for studying the role of GM-CSF in various biological processes.
Automatically generated - may contain errors
3 protocols using csf2 mice
1
Transgenic Mouse Strains for Research
2
Murine Model Characterization Protocol
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Four- to six-week-old male WT C57BL/6 mice and Csf2-/- mice were purchased from The Jackson Laboratory (Bar Harbor, ME). The original Mt1/2-/- strain (129S7/SvEvBrd-Mt1tm1BriMt2tm1Bri/J) was backcrossed >9 times to C57BL/6J mice, and genetic analysis revealed them to be >99% C57BL/6J. All animals were housed under specific pathogen-free conditions in the University of Cincinnati’s Department of Laboratory Animal Medical Services facility, which is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care. All experiments were performed in accordance with the Animal Welfare Act guidelines of the National Institutes of Health.
3
Comparative Study of WT and CSF2-/- Mice
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C57BL/6 WT and CSF2−/− mice were purchased originally from Jackson Laboratory (Bar Harbor, Maine). Animals were housed in specific pathogen-free conditions with an alternating light/dark cycle. All experiments were performed using 6- to 8-week-old male mice. Care, use and treatment of mice in this study were in strict agreement with international guidelines for the care and use of laboratory animals and approved by Animal Ethics Committee of Jilin University.
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