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Extensograph e

Manufactured by Brabender
Sourced in Germany

The Extensograph-E is a lab equipment product that measures the extensibility and resistance to extension of dough samples. It determines the rheological properties of dough, providing data on its viscoelastic behavior. The Extensograph-E records the force required to stretch a dough sample over time, generating a standardized extensogram curve.

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6 protocols using extensograph e

1

Flour Composition and Rheological Properties

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Before steamed bread preparation, chemical composition and rheological properties of the potato–wheat formulated flour samples (S0, S5, S10, S15, S20, S25, S30, S35, and S40) and basic flour–gluten formulated flour samples (S35+W6.0, S35+W6.5, and S35+W7.0) were tested. Moisture, ash, protein, and gluten index were determined according to the AACC methods 44‐15A, 08‐21.01, 46‐12.01, and 38‐12.02, respectively (AACC, 2000). Farinograph test was performed according to the ICC method No.115/1 by Farinograph‐AT (Brabender) (ICC, 2010). Extensograph test was carried out according to the ICC method No. 114/1 by Extensograph‐E (Brabender) (ICC, 2010).
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2

Comprehensive Wheat Quality Analysis

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Samples of 39 wheat accessions from Henan Province, China were milled following American Association of Cereal Chemists (AACC) approved method 26–21.02 utilizing Brabender Quadrumat Junior Mill (Brabender, Germany). The moisture and protein content test of flour was conducted based on AACC approved method 46–30.01 utilizing IM9500 (Perten, Sweden). The wet gluten content test of flour was conducted based on AACC approved method 38–12A utilizing GM2200 (Perten, Sweden). The Farinograph test of flour was conducted based on AACC approved method 54–21.02 utilizing Farinograph-E (Brabender, Germany). The Extensograph test of flour was conducted based on AACC approved method 5410 utilizing Extensograph-E (Brabender, Germany). In addition, the whole-wheat meal of 39 samples obtained utilizing LM3100 (Perten, Sweden) were tested by GlutoPeak. Each test was replicated three times, and the average values were used for further analysis.
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3

Dough Rheological Properties Evaluation

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Changes in the consistency and elasticity of the dough during its formation and mixing were determined by means of a farinograph (Farinograph-E, Brabender, Duisburg, Germany), in accordance with the PN-ISO 5530-1:1999 standard [11 ]. As a part of this analysis, the water absorption of flour (%), dough development time (min), dough stability (min), degree of softening (FU) after 10 min, and farinographic number were determined. The analysis was performed in triplicate. In turn, the analysis of the dough extensibility was carried out using an extensograph (Extensograph-E, Brabender, Duisburg, Germany) in accordance with the PN-ISO 5530-2: 2004 standard [12 ]. As a part of this test, the energy of the dough (cm2), resistance of extension (BU), extensibility (mm), maximum (BU), and ratio number were determined. The analysis was performed in duplicate.
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4

Farinographic and Extensographic Analyses of Flour

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The farinographic properties of all samples were determined in a farinograph (Farinograph-E, Brabender, Duisburg, Germany) [7 (link),10 ]. The time of analysis was 15 min. The parameters evaluated were flour water absorption (WA) [%], dough development time (DDT) [min], dough stability [min], degree of softening [BU] and farinographic number. In addition, the extensographic characteristics were analyzed in a Brabender extensograph (Extensograph-E, Brabender, Duisburg, Germany) [7 (link),11 ]. In this study, dough energy [cm2], resistance to extension [BU], extensibility [mm], maximum [BU] and ratio number were determined.
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5

Extensographic Analysis of Wheat Flour and Protein Isolate Blends

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The extensographic analysis was performed on a Extensograph-E instrument (Brabender, Germany) heated to 30 °C. The instrument settings are listed in Table 2. The extensographic measurement was conducted on T530 flour, T530 flour with 5, 10, and 15% substitution of flour with WBPI and WPI.
Dough was prepared using a mixer, which was part of the farinograph. Additionally, 6 g of sodium chloride were weighed and dissolved in the appropriate amount of water to prepare 0.1 M solution. This solution was used to prepare the dough of 400 FU consistency. The optimized amount of water for each mixture is provided in Table 3.
The dough was formed into a small ball using a shaper and then placed into a sheeter and reshaped into a cylinder. Dough cylinders were placed into a chamber heated to 30 °C and left to rest for 30 min. After the resting period, the dough was stretched by the device’s hook until it broke after which it was kneaded again, reshaped in the sheeter, and allowed to rest for another 30 min. Experiment was repeated. The resistance exerted by the hook on the dough during stretching was recorded.
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6

Extensional Dough Properties Evaluation

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Extensional tests were performed using an extensograph-E (Brabender, Duisburg, Germany) equipped with a Micro-extensograph unit for 20 g dough pieces. Doughs were prepared with a DoughLab in a 50 g bowl according to Farinograph WA using 126 rpm to a total energy input of 12 Wh/kg. Bowl temperature was set at 24 • C to ensure a final dough temperature of 30 • C. Tests were otherwise performed according to AACC method 54-10. Extensibility and resistance to extension were recorded (n = 3 independent doughs).
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