Tumor necrosis factor-α (TNF-α), interleukin (IL)-17A, IL-10, IL-6 enzyme-linked immunosorbent assay (ELISA) kits, fluorescent-labeled monoclonal antibodies anti-CD4-fluorescein isothiocyanate (FITC), anti-IgG1-FITC, anti-CD25-Phycoerythrin (PE), anti-IgG1-PE, anti-FoxP3-Allophycocyanin (APC), anti-Interferon (IFN)-gamma-PE, anti-IgG1-APC, and anti-IL-17-APC were bought from eBiosciences (San Jose, CA, USA). FIX & PERM medium was obtained from Invitrogen (California, USA). Leukocyte Activation Cocktail was obtained from BD (New York, USA), Catalog No.550583, containing phorbol 12-myristate 13-acetate (PMA), a calcium ionophore (ionomycin), and the protein transport inhibitor BD GolgiPlug™ (Brefeldin A, New York, USA).
Anti cd25 phycoerythrin pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-CD25-Phycoerythrin (PE) is a fluorescently labeled antibody that binds to the CD25 antigen. CD25 is expressed on activated T cells, B cells, and natural killer cells. The phycoerythrin (PE) fluorescent dye is conjugated to the antibody, which allows for the detection and quantification of CD25-positive cells using flow cytometry or other fluorescence-based methods.
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Lab products found in correlation
Golgiplug, by BD (1 mentions)
Anti foxp3 allophycocyanin apc, by Thermo Fisher Scientific (1 mentions)
Leukocyte activation cocktail, by BD (1 mentions)
Anti il 17 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd4 fluorescein isothiocyanate fitc, by Thermo Fisher Scientific (1 mentions)
Anti igg1 pe, by Thermo Fisher Scientific (1 mentions)
Fix perm medium, by Thermo Fisher Scientific (1 mentions)
Anti cd4 fitc, by Thermo Fisher Scientific (1 mentions)
Anti foxp3 apc, by Thermo Fisher Scientific (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Fixation permeabilization kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd25 phycoerythrin pe
1
Cytokine and Lymphocyte Profiling Protocol
2
Quantification of Regulatory T Cells
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Blank controls of cultured T cells, isolated PBMCs, and splenic MNCs were treated the same as samples, except for incubation with fluorescent antibody. Isotype controls of isolated PBMCs and splenic MNCs were treated the same as blank controls but were incubated with rat IgG2a kappa isotype control (Ebr2a) and allophycocyanin (APC) (eBioscience) after fixation/permeabilization. Cultured T cells, isolated PBMCs, and splenic mononuclear cells were incubated with fluorescent antibody to test the percentages of Tregs (CD4+CD25+Foxp3+). To stain CD4, CD25, and Foxp3, anti-CD4-FITC, anti-CD25-phycoerythrin (PE), anti-Foxp3-APC, and a Fixation/Permeabilization kit (eBioscience) were used according to the manufacturer's instructions. At least 105 cells were collected with a CytoFLEX flow cytometer (Beckman Coulter, Pasadena, CA, USA) and were analysed with FlowJo software V10.
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