Log In Sign up
The largest database of trusted experimental protocols

Stat antibody sampler kit

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in China, United States

The Stat Antibody Sampler Kit is a collection of antibodies targeting various members of the Signal Transducer and Activator of Transcription (STAT) family of proteins. The kit includes primary antibodies specific to STAT1, STAT3, STAT4, STAT5A, STAT5B, and STAT6.

Automatically generated - may contain errors

Lab products found in correlation

P stat antibody sampler kit, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Phospho jak3, by Cell Signaling Technology (1 mentions) Phospho stat4, by Cell Signaling Technology (1 mentions) Phospho stat2, by Cell Signaling Technology (1 mentions) Phospho jak2, by Cell Signaling Technology (1 mentions) Phospho stat1, by Cell Signaling Technology (1 mentions) Chemiluminescent detection reagents ecl, by Cytiva (1 mentions) F3648, by Merck Group (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Ab34710, by Abcam (1 mentions) Ab25117, by Abcam (1 mentions) Anti β catenin, by BD (1 mentions) Anti active β catenin, by Cell Signaling Technology (1 mentions) Anti p gsk3β ser9, by Cell Signaling Technology (1 mentions) Ab5694, by Abcam (1 mentions) Ab180714, by Abcam (1 mentions) Recombinant mouse il 4, by R&D Systems (1 mentions) Sepharose tm 4 fast flow, by GE Healthcare (1 mentions) Sp600125, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-Stat Antibody Sampler Kit (3 citations) P‐STAT antibody sampler kit (2 citations)

4 protocols using stat antibody sampler kit

1

Signaling Pathway Characterization by Western Blot and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting, cell counting kit 8 (CCK‐8), Transwell migration, and flow cytometry assays were performed as previously described (Patel et al., 2006). The primary antibodies used in this study were as follows: GAPDH (dilution 1:1000; Cell Signaling Technology, Inc.), XCR1 (dilution 1:600; Cell Signaling Technology, Inc.), JAK1 (dilution 1:600; cat. no. 2019; Cell Signaling Technology, Inc.), phospho‐JAK1 at Tyr1034/1035 (dilution 1:500; Cell Signaling Technology, Inc., JAK2 (dilution 1:1000; Cell Signaling Technology, Inc.), phospho‐JAK2 at Tyr1007 (dilution 1:1000; Cell Signaling Technology, Inc.), JAK3 (dilution 1:500; Cell Signaling Technology, Inc.), phospho‐JAK3 at Tyr980 (dilution 1:500; Cell Signaling Technology, Inc.), STAT 1–4 (dilution 1:500; dilution 1:500; Stat Antibody Sampler Kit, Cell Signaling Technology, Inc.), phospho‐STAT1 at Tyr701, phospho‐STAT2 at Tyr690, and phospho‐STAT4 at Tyr693 (dilution 1:500; Cell Signaling Technology, Inc.).
Chang X., Cao Y., Fu W., Tang X., Wang Y., Lv Y, & Guo Q. (2020). Overexpression of chemokine receptor lymphotactin receptor 1 has prognostic value in clear cell renal cell carcinoma. Molecular Genetics & Genomic Medicine, 9(1), e1551.
+ Open protocol
+ Expand
2

Western Blotting Analysis of Key Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously(34 (link)). cTAGE1 and Rec8 rabbit polyclonal antibodies were purchased from Proteintech (Chicago, IL). GTSF1 rabbit polyclonal antibody was purchased from Abnova (Walnut, CA). SYCP1 rabbit polyclonal antibody was purchased from GenTex (Irvine, CA). SPO11 rabbit polyclonal antibody was purchased from Abcam (Cambridge, MA). STAT antibodies were purchased from Cell Signaling (Danvers, MA) as part of Stat Antibody Sampler kit (Catalog #9939). Also, STAT3 (79D7) Rabbit mAb (Catalog number #4904) was used in our Western Blot experiements. Chemiluminescent detection reagents (ECL) were purchased from Amersham Biosciences (Piscataway, NJ).
Litvinov I.V., Cordeiro B., Huang Y., Zargham H., Pehr K., Doré M.A., Gilbert M., Zhou Y., Kupper T.S, & Sasseville D. (2014). Ectopic expression of cancer testis antigens in Cutaneous T-Cell Lymphoma (CTCL) patients. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(14), 3799-3808.
+ Open protocol
+ Expand
3

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein expression levels were analysed by Western blot analysis as described previously.15, 18 The primary antibodies used were as follows: anti‐SDF‐1α (ab25117; Abcam), anti‐fibronectin (F3648; Sigma‐Aldrich), anti‐α‐SMA (ab5694; Abcam), anti‐collagen I (ab34710; Abcam), anti‐vimentin (SAB1305447, Sigma‐Aldrich), anti‐CXCR4 (BA0747‐2; Boster), anti‐p‐GSK3β (ser 9) (5558; Cell Signaling Technology), anti‐β‐catenin (610 154; BD Transduction Laboratories), anti‐PAI‐1 (AF3828; R&D Systems), anti‐snail1 (ab180714; Abcam), anti‐MMP‐7 (104 658; GTX), anti‐E‐cadherin (3195; Cell Signaling Technology), anti‐active β‐catenin (19807s; Cell Signaling Technology), anti‐Kim1 (BA3537; Boster, Wuhan, China), anti‐α‐tubulin (RM2007, Ray Antibody Biotech, Beijing, China) and p‐STAT antibody sampler kit (9914; Cell Signaling Technology), p‐JAK family antibody sampler kit (97 999; Cell Signaling Technology), STAT antibody sampler kit (9939; Cell Signaling Technology) and anti‐GAPDH (RM2000, Ray Antibody Biotech, Beijing, China).
Liu Y., Feng Q., Miao J., Wu Q., Zhou S., Shen W., Feng Y., Hou F.F., Liu Y, & Zhou L. (2020). C‐X‐C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3β/β‐catenin pathway. Journal of Cellular and Molecular Medicine, 24(7), 3837-3855.
+ Open protocol
+ Expand
4

Polyclonal Antibody Generation for Tmem106a

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal antibodies against mouse Tmem106a were prepared by immunizing rabbits with chemically synthesized Tmem106a peptides (Figure S1, boxed sequences), purified by peptide affinity chromatography with CNBr-activated SepharoseTM 4 Fast Flow (GE Healthcare, 17-0981-01), according to the manufacturer’s instructions. Other primary antibodies used in this study were: anti-NF-κB (p65), ERK-1/2, JNK, p38 MAPK, phosphor (p)-ERK-1/2 (Thr202/Tyr204), p-JNK (Thr183/Tyr185), p-38MAPK (Thr180/Tyr182), p-NF-κB (p65), Stat Antibody Sampler Kit and p-Stat Antibody Sampler Kit (all purchased from Cell Signaling Technology, Beverly, MA, USA). U0126, SB203580 and SP600125 were also from Cell Signaling Technology. Recombinant mouse IL-4 was from R&D Systems (MN, USA). Horseradish peroxidase or FITC-conjugated anti-rabbit-IgG antibodies were from Southern Biotechnology Associates Inc. (Birmingham, AL, USA).
The followings are the sequences of double-stranded siRNAs against Tmem106a, which were designed and chemically synthesized by Genechem Corporation (Shanghai, China): siTmem106a-1: 5'-GCA CAC UCC UGU AUG CCU UTT-3'; siTmem106a -2: 5'-GGU UGC UCU CAU CCC UUA UTT-3'. The non-silencing control siRNA was confirmed to have no matches with the complete mouse genome by a BLAST search (www.ncbi.nlm.nih.gov).
Dai H., Xu D., Su J., Jang J, & Chen Y. (2015). Transmembrane protein 106a activates mouse peritoneal macrophages via the MAPK and NF-κB signaling pathways. Scientific Reports, 5, 12461.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!