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Stepone plus real time pcr detection thermal cycler

Manufactured by Thermo Fisher Scientific
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The StepOne-Plus Real-Time PCR detection thermal cycler is a laboratory instrument designed for real-time polymerase chain reaction (PCR) amplification and detection. It provides a platform for quantitative analysis of DNA and RNA samples.

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Lab products found in correlation

Qpcr rt kit, by Toyobo (2 mentions) Sybr green realtime pcr master mix, by Toyobo (2 mentions)

Close spelling variants of this product

StepOne Real-Time PCR detection thermal cycler StepOne Plus real-time cycler Real Time PCR StepOne Plus StepOne Plus thermal cycler StepOne Plus PCR cycler StepOne Plus Real Time StepOne Real-Time PCR StepOne thermal cycler PCR thermal cycler StepOne cycler

2 protocols using stepone plus real time pcr detection thermal cycler

1

Quantitative Expression Analysis of Larval Organs

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At least 20 larval organs (at least 100 for the lymph gland) were dissected to extract RNA. cDNA was synthesized with qPCR-RT kit (TOYOBO). qRT-PCR was performed by comparative CT method using SYBR Green Realtime PCR Master Mix (TOYOBO) and a StepOne-Plus Real-Time PCR detection thermal cycler (Applied Biosystems). Specific primers used for qRT-PCR are described in Supplementary Table 2.
Cho B., Spratford C.M., Yoon S., Cha N., Banerjee U, & Shim J. (2018). Systemic control of immune cell development by integrated carbon dioxide and hypoxia chemosensation in Drosophila. Nature Communications, 9, 2679.
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2

Hemocyte mRNA Extraction and RT-qPCR Analysis

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At least 100 larvae were dissected to extract hemocyte mRNA, and cDNA was synthesized with a qPCR RT kit (TOYOBO). The RT-qPCR was performed by the comparative Ct method using SYBR Green Realtime PCR Master Mix (TOYOBO) and a StepOne-Plus Real-Time PCR detection thermal cycler (Applied Biosystems). Nine primer pairs were used for this analysis (F means forward, R means reverse, and all primers are written in the 5’ to 3’ direction):
Yoon S.H., Cho B., Lee D., Kim H., Shim J, & Nam J.W. (2023). Molecular traces of Drosophila hemocytes reveal transcriptomic conservation with vertebrate myeloid cells. PLOS Genetics, 19(12), e1011077.
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