Ab122241

For Western blot analysis, the following antibodies were used in a 1:1000 dilution: anti-β (ab14730, Abcam), anti-γ (PA5-29975, ThermoFisher), anti-b (ab117991, Abcam), anti-OSCP (ab110276, Abcam), anti-f (ab200715, Abcam), anti-g (ab126181, Abcam), anti-e (ab122241, Abcam), anti-c (ab181243, Abcam), anti-a (ab192423, Abcam), anti-citrate synthase (ab96600, Abcam), anti-vinculin (V4505, Sigma), anti-prohibitin (MS-261-P1, NeoMarkers), anti-GAPDH (2118, Cell Signaling), anti-CyPD (ab110324, Abcam), anti-ANT2 (14671, Cell Signaling), anti-ANT3 (PA5-35113, ThermoFischer), anti-OXPHOS (ab110411, Abcam), anti-HKII (sc130358, Santa Cruz), anti-UQCRC1 (sc65238, Santa Cruz), anti-Grim19 (sc271013, Santa Cruz), and anti-SDHA (sc166947, Santa Cruz). Statistical comparison of data was assessed with the two-sided Student’s t-test.

Primary antibodies were anti-UQCR polyclonal antibody (14793-1-AP; Proteintech) and anti-ATP5I polyclonal antibody (ab122241; Abcam). Antigen retrieval was performed according to the manufacturer’s instructions. Numbers of UQCR7-positive tufts per glomerulus were counted in more than ten glomeruli per kidney section. The relative amounts of UQCR7 staining in vascular poles of glomeruli were also scored semi-quantitatively in more than ten glomeruli per kidney section as follows: 0 = negative staining, 1 = positive in less than 50% of the circumference of vascular pole, 2 = positive in equal to or more than 50% of the circumference 3 = positive in whole circumference. In addition, percentages of UQCR7-positive cells in distal tubular cells were calculated by counting more than fifty distal tubular cells per section. Positive rates of Atp5I in tubular cells were evaluated.