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2690 alliance hplcseparation module

Manufactured by Waters Corporation

The 2690 Alliance HPLC separation module is a high-performance liquid chromatography (HPLC) system designed for laboratory use. It provides reliable and consistent separation of complex mixtures. The module incorporates a solvent delivery system, an autosampler, and a temperature-controlled column compartment to enable efficient and reproducible chromatographic separations.

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2 protocols using 2690 alliance hplcseparation module

1

Analytical Techniques for Biomolecular Characterization

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Ultraviolet–visible
(UV–vis) spectrophotometer measurements were performed using
a SpectraMax M5 multimode plate reader (Molecular Devices, San Jose,
CA). HPLC measurements were carried out using a 2690 Alliance HPLC
separation module coupled with a 2487 dual λ absorbance UV–vis
detector (Waters, Milford, MA). Analytes were weighed using a Mettler
Toledo XS104 analytical balance (Mettler-Toledo, Columbus, OH). Fluorescence
spectroscopy measurements were performed using a SpectraMax M5 multimode
plate reader (Molecular Devices, San Jose, CA).
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2

HPLC Analytical Method Development

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HPLC measurements were carried out on 2690 Alliance HPLC separation module coupled with 2487 dual λ absorbance detector (Waters, Milford, MA). Analytes and internal standard (IS) were weighed using Mettler Toledo XS104 analytical balance (Mettler-Toledo, LLC, Columbus, OH). UV absorbance spectrum was collected using SpectraMax Plus384 microplate spectrophotometer (Molecular Devices, Sunnyvale, CA). The extracted plasma samples were dried using TurboVap LV (Caliper Life Sciences, Inc, Waltham, MA) under a nitrogen stream.
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