Architect c18000 analyzer

Manufactured by Abbott

Sourced in United States

The Architect C18000 analyzer is a high-throughput automated clinical chemistry and immunoassay analyzer. It is designed to perform a variety of laboratory tests, including clinical chemistry, immunoassay, and special chemistry tests.

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Architect analyzer (56 citations)

3 protocols using architect c18000 analyzer

Plasma Glucose and Lipid Profile

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Plasma glucose and lipid profile were measured on a commercial analytical system (Architect C18000 analyzer, Abbott Laboratories, Illinois, USA). Hs-CRP was quantified by immunoturbidimetric assay, insulin, leptin and PRA by radio-immuno assay (Linco Research, Inc, Missouri, USA; REN-CT2, CIS bio international, France), and NEFA by enzymatic colorimetry (Wako Pure Chemical Industries, Ltd, Osaka, Japan).

Straznicky N.E., Grima M.T., Sari C.I., Eikelis N., Nestel P.J., Dixon J.B., Lambert G.W., Schlaich M.P., Phillips S.E, & Lambert E.A. (2017). Neck Circumference Is Associated with Muscle Sympathetic Nerve Activity in Overweight and Obese Men but Not Women. Frontiers in Physiology, 8, 203.

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Biochemical Profiling of Plasma Markers

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Plasma glucose and lipid profile were measured on a commercial analytical system (Architect C18000 analyzer, Abbott Laboratories, Illinois, USA). High sensitivity C-reactive protein (hs-CRP was quantified by immunoturbidimetric assay, insulin and leptin by radio-immuno assay (Linco Research, Inc, Missouri, USA), C-peptide by chemiluminescent immunoassay (ADVIA Centaur, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and non-esterified fatty acids (NEFA) by enzymatic colorimetric method (Wako Pure Chemical Industries, Ltd, Osaka, Japan). Plasma norepinephrine was assayed by high performance liquid chromatography with electrochemical detection, following extraction by alumina adsorption. [³H]-norepinephrine was assayed by liquid scintillation chromatography and corrected for loss during extraction using recoveries of internal standard. Intra-assay CVs in our laboratory are 1.3 % for norepinephrine and 2.3 % for [³H]-norepinephrine; inter-assay CVs are 3.8 and 4.5 %, respectively.

Straznicky N.E., Grima M.T., Sari C.I., Lambert E.A., Phillips S.E., Eikelis N., Kobayashi D., Hering D., Mariani J.A., Dixon J.B., Nestel P.J., Karapanagiotidis S., Schlaich M.P, & Lambert G.W. (2015). Reduction in peripheral vascular resistance predicts improvement in insulin clearance following weight loss. Cardiovascular Diabetology, 14, 113.

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Biomarker Measurement Protocols

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Plasma glucose and lipid profile were measured on a commercial analytical system (Architect C18000 analyzer, Abbott Laboratories, Illinois, USA). High sensitivity C-reactive protein (hs-CRP) was quantified by immunoturbidimetric assay, insulin, leptin, and PRA by radio-immuno assay (Linco Research, Inc., Missouri, USA; REN-CT2, CIS bio international, France) and NEFA by enzymatic colorimetry (Wako Pure Chemical Industries, Ltd, Osaka, Japan). These assays were performed in duplicate. Plasma norepinephrine was assayed by high performance liquid chromatography with electrochemical detection, following extraction by alumina adsorption. [³H]-norepinephrine was assayed by liquid scintillation chromatography and corrected for loss during extraction using recoveries of internal standard. Intra-assay CVs in our laboratory are 1.3% for norepinephrine and 2.3% for [³H]-norepinephrine; inter-assay CVs are 3.8 and 4.5%, respectively.

Straznicky N.E., Grima M.T., Sari C.I., Lambert E.A., Phillips S.E., Eikelis N., Mariani J.A., Kobayashi D., Hering D., Dixon J.B, & Lambert G.W. (2016). Comparable Attenuation of Sympathetic Nervous System Activity in Obese Subjects with Normal Glucose Tolerance, Impaired Glucose Tolerance, and Treatment Naïve Type 2 Diabetes following Equivalent Weight Loss. Frontiers in Physiology, 7, 516.

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