2 4 6 trinitrobenzene sulfonic acid

Erlotinib hydrochloride and O-desmethyl erlotinib were obtained from AdooQ BioScience LLC (Irvine, CA, USA). Ethyl 3,4-bis(2-methoxyethoxy)benzoate (EBMB) was obtained from AK Scientific, Inc (Union City, CA, USA). 2,4,6-Trinitrobenzene sulfonic acid was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Horseradish peroxidase (HRP) and 3,3,5,5-tetramethylbenzidine (TMB) were obtained from Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). Sodium hydroxide, hydrochloric acid, sulfuric acid, sodium dihydrogenphosphate dehydrate, disodium hydrogenphosphate 12-water, sodium azide, tris(hydroxymethyl)aminomethane (Tris), 1-ethyl-3,3-dimethylaminopropyl carbodiimide hydrochloride (EDC), N-hydroxysuccinimide, dioxane, ethyl acetate, N,N-dimethylformamide and bovine serum albumin (BSA) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Hydrogen peroxide (30% in water) was obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Afatinib was obtained from ChemScene LLC (Monmouth Junction, NJ, USA). (S)-N-4-(3-Chloro-4-fluorophenyl)-7-(tetrahydrofuran-3-yloxy)-quinazoline-4,6-diamine (CTQD) was obtained from Toronto Research Chemicals, Inc. (Toronto, ON, Canada). N-4-(3-Chloro-4-fluorophenyl)-7-methoxyquinazoline-4,6-diamine (CMQD) was obtained from Ark Pharm, Inc. (Libertyville, IL, USA). Vandetanib was obtained from AdooQ BioScience LLC (Irvine, CA, USA). 2,4,6-Trinitrobenzene sulfonic acid was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Keyhole limpet hemocyanin (KLH) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Horseradish peroxidase (HRP) and 3,3′,5,5′-tetramethylbenzidine (TMB) were obtained from Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). The other reagents and solvents were all of the highest commercially available grade.

Whey proteins powder was kindly gifted by Asama Chemical Co. Ltd. (Tokyo, Japan). Dimedone and 2,4,6-Trinitrobenzenesulfonic acid (TNBS) were bought from Tokyo Chemical Industry (Tokyo, Japan), and Alexa 680 Fluor C2 Maleimide was from Thermo Scientific (Rockford, IL, USA). Anti-cysteine sulfenic acid antibody was obtained from Millipore (MA, USA). HRP- conjugated anti-rabbit or mouse IgG, anti-β-actin, and anti-caspase-3 antibodies were purchased from Cell Signaling, Inc. (Beverly, MA, USA). A blood urea nitrogen (BUN) kit was purchased from Thermo Fisher Scientific (Frederick, MD, USA). Anti-lipocalin-2 antibody was obtained from Adipogen (MI, USA). E-cadherin were bought from BD Biosciences, (NJ, USA). Cytokines IL-1α, IL-1β, and TNF-α assay kits were purchased from PeproTech (Rocky Hill, NJ, USA). ROS and superoxide were from Enzo life science (NY, USA), NOX-2 antibody was brought from Bioss Antibodies (MA, USA) and NOX-4 antibody was purchased from Novus Biologicals (CO, USA), H₂O₂ detection kit was from Cayman Chemical Company (MI, USA), Calcein-AM and Propidium Iodide (PI) Staining Kit was from Dojindo Molecular Technologies (MD, USA). Pentobarbital sodium was from Kyoritsu Seiyaku Corporation (Tokyo, Japan). Maleimide and all other chemicals were bought from Sigma-Aldrich (St. Louis, MO, USA).