Nano zs laser particle size analyzer

TEM was carried out with a Hitachi H–7500 microscope operated at 80 kV. UV-vis absorption spectra were performed using on a Hewlett–Packard 8452 diode array spectrometer (U–3010). XPS spectra were obtained by an ESCALAB 250 X-ray photoelectron spectrometer (Axis Ultra DLD, UK). Particle size distribution was recorded by Malvern Nano-ZS Laser Particle Size Analyzer (UK). SERS spectra were acquired using a confocal microprobe Raman system (LabRAM Aramis, France) operated with a He–Ne laser (632.8 nm) and the data acquisition times were 10 and 20 s for the Au NAs and isolated NPs, respectively. The SERS samples were prepared by adding 3.8 μL of 10^–4 M R6G into 15 μL of 4.9 × 10^–9 M isolated NPs and 111, 212 and 222 Au NAs, respectively, and then the mixed solutions were dropped and dried on glass for SERS measurements.

A Nano ZS laser particle size analyzer (Malvern, UK) was used to monitor the particle size distribution of the obtained nonaqueous suspensions in Section 4.5.1., which were left at different temperatures (30 °C, 80 °C, 130 °C) to rest in a bottle for 1 week.