Acetylcholinesterase (AChE) from electric eels, acetylthiocholine chloride (ATCh), potassium ferricyanide (K
3Fe(CN
6), 99%) and
chlorpyrifos were purchased from Sigma-Aldrich Co, Ltd, China.
Hydrochloric acid (HCl, 37%),
sodium chloride (NaCl, 99%), potassium chloride (KCl, <100%),
acetone (CH
3COOH
3, ≥99.8%), and
ethanol (C
2H
5OH, ≥99.7) were purchased from RCI Labscan, Thailand. Magnesium nitrate (Mg(NO
3)
2, ≥99.4%) and cobalt(
ii) nitrate hexahydrate (Co(NO
3)
2·6H
2O, ≥98%) were purchased from Univar, Australia.
Calcium chloride (CaCl
2, ≥95%) was purchased from Scharlau, Spain. Mercury(
ii) chloride (HgCl
2, ≥99.5%) was purchased from QRec™, New Zealand. Chitosan ((C
6H
11O
4)
n, 100 000–300 000) was purchased from ACROS, China. Phosphate buffer saline (PBS, 1× concentration, osmolarity 280–315 mOsm kg
−1) was purchased from Cepham Life Science, USA. Hydrogen peroxide (H
2O
2, 30%, ISO grade) was purchased from Merck Millipore, USA. Deionized water (DI) with specific resistivity of 18.2 MΩ cm was obtained from a RiOs™ Type I Simplicity 185 Millipore water purification system. Alkaline hydrogen peroxide (AHP) solution was prepared by adding 1 N NaOH to a 3% H
2O
2 solution, adjusted to a pH of 11.5. Water and juices were bought from a convenience store in Khon Kaen, Thailand. Sugarcane bagasse was bought from a local market in Khon Kaen, Thailand.
Thet Tun W.S., Saenchoopa A., Daduang S., Daduang J., Kulchat S, & Patramanon R. (2023). Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice. RSC Advances, 13(14), 9603-9614.