Sterile alginate powder (Sigma) was added to sterile saline to create a 2% w/v solution. The final concentration of tmSCF nanodiscs was adjusted to 50 µg/ml in this 2% alginate solution. To prepare the crosslinker, calcium sulfate was added to sterile saline to create 0.2% w/v solution. Both the alginate solution and the crosslinker were taken up into a 1 ml syringe just before injection (100 μl of each solution for 200 μl total per injection).
Sterile alginate powder
Manufactured by Merck Group
Sterile alginate powder is a laboratory product used as a starting material for various applications. It is a naturally derived polysaccharide with a wide range of functional properties. The powder is sterilized to ensure a high level of purity and safety for use in controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sterile alginate powder
1
Alginate Hydrogel for Nanodiscs Delivery
2
Alginate Hydrogel Encapsulation of Nanodiscs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sterile alginate powder (Sigma) was added to sterile saline to create a 2% w/v solution. The final concentration of tmSCF nanodiscs was adjusted to 50 μg/ml in this 2% alginate solution. To prepare the crosslinker, calcium sulfate was added to sterile saline to create 0.2% w/v solution. Both the alginate solution and the crosslinker were taken up into a 1 ml syringe just before injection (100 μl of each solution for 200 μl total per injection).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!