Two groups of mice, ApoE-KO (n = 8) and controls (n = 8), were used for in vivo imaging studies. Mice were kept fully sedated with 1.5–2% isoflurane during injections and SPECT/CT imaging. SPECT/CT images were acquired using nanoSPECT/CT (Mediso medical imaging systems, Hungary) with four detectors after i.v. injection with 2–3 MBq of 111In-tilmanocept (0.4–0.6 nmol/mouse), 90 min p.i. Ultra-high resolution (0.6 mm) multi-pinhole whole-body mouse collimators were used. In addition, 20 angular projections with 40 s/projection and a multi-peak energy window (245 keV ± 20% and 171 keV ± 20%) were used. A group of ApoE-KO mice was co-injected with blocking dose (10 nmol) of non-labelled tilmanocept. Data were reconstructed using TeraTomo software (Mediso medical imaging systems, Hungary), and a 3D Monte Carlo-based algorithm was used (64 × 64 matrix, voxel size in 0.47 × 0.47 × 0.47 mm3, 3 subsets and 48 iterations).
Teratomo software
Manufactured by Mediso
Sourced in Hungary
TeraTomo is a software solution developed by Mediso for processing and visualizing tomographic data. It provides tools for reconstructing, analyzing, and managing large-scale tomographic datasets. The software's core function is to enable efficient handling and interpretation of complex volumetric imaging data.
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Lab products found in correlation
2 protocols using teratomo software
1
In vivo SPECT/CT Imaging of ApoE-KO Mice
2
In vivo SPECT/CT Imaging of Radiolabeled Nanoparticles
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The animal study was performed in respect of the applicable regulation for animal experimentation and with approval of the animal care and use committee of the Claude Bernard Institute (Autor. APAFIS #8724, Paris, France). C57BL/6 male mice (weight 20-23 g) (EJ, Le Genest, St-Berthevin, France) were used for in vivo imaging studies. Mice were kept fully sedated with 1.5-2% isoflurane during the injection step and the SPECT/CT imaging. 200 µL of the radiolabeled suspension (30 MBq) corresponding to 100 µg of nanoparticles was injected through the retro-orbital route. SPECT/CT images were acquired 80 minutes after injection, using a nanoSPECT/CT apparatus (Mediso medical imaging systems, Hungary) with 4 detectors. Ultra-high resolution (0.5 mm) multipinhole whole body mouse collimators were used. In addition, 20 angular projections with 50 s/projection and a peak energy window of 140 keV ± 20% were used. Data were reconstructed using the TeraTomo software (Mediso medical imaging systems, Hungary) and a 3D Monte Carlo-based algorithm (64×64 matrix, voxel size of 0.47×0.47×0.47 mm 3 , 3 subsets and 48 iterations). Finally, slides were visualized in 3 planes: sagittal, coronal and axial. A 3D movie was also reconstructed.
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