Cells were lysed with lysis buffer (100 mM Tris–HCl, pH 6.8, 100 mM DTT, 1% SDS, 10% glycerol). Proteins were separated by 10–12% SDS-PAGE, and transferred to PVDF membranes. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed and blocked with 5% milk and incubated with different primary antibodies overnight at 4 °C, followed by incubation with secondary antibodies. The primary antibodies used in western blotting included anti-Fos B(F-7) (sc-398595; Santa Cruz; 1:1000 dilution), anti-CEBPB (sc-7962; Santa Cruz; 1:1000 dilution), anti- histone H3K27me3 (ab6002, Abcam; 1:1000 dilution), anti-histone H3K27ac (#8173, Cell Signaling; 1:1000 dilution) and anti-GAPDH (sc-47724; Santa Cruz; 1:5000).
Anti fos b f 7
Manufactured by Santa Cruz Biotechnology
Anti-Fos B(F-7) is a mouse monoclonal antibody that recognizes the Fos B protein. Fos B is a transcription factor that plays a role in cellular processes such as cell growth and differentiation. The antibody can be used for the detection and study of Fos B expression in various experimental systems.
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Lab products found in correlation
2 protocols using anti fos b f 7
1
Western Blot Analysis of Epigenetic Markers
2
Western Blot Analysis of FosB Protein
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Cells were lysed in RIPA buffer and boiled for 10 min. Protein samples were separated by 10% SDS-PAGE and transferred to PVDF membranes. Following protein transfer, the membranes were blocked with 5% milk and incubated with different primary antibodies overnight at 4℃, followed by incubation with secondary antibodies. The primary antibodies used in western blotting included anti-Fos B(F-7) (sc-398595, Santa Cruz; 1:1000 dilution ) and anti-GAPDH (sc-47724; Santa Cruz; 1:5000).
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