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Alexa fluor 647 mouse anti pstat5y694 clone 47

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Alexa Fluor 647 mouse anti-pSTAT5Y694 (clone 47) is a fluorescently labeled antibody that binds to the phosphorylated form of STAT5 at tyrosine 694. It can be used to detect and quantify the level of phosphorylated STAT5 in cells.

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Lab products found in correlation

Lsrfortessa cell analyzer, by BD (2 mentions) Phosflow perm buffer 3, by BD (2 mentions) Phosflow fix buffer 1, by BD (2 mentions)

Close spelling variants of this product

Alexa Fluor 647 Alexa 647 Mouse anti

2 protocols using alexa fluor 647 mouse anti pstat5y694 clone 47

1

Quantifying STAT5 Phosphorylation in AML

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was performed with Alexa Fluor 647 mouse anti-pSTAT5Y694 (clone 47, BD Biosciences). MOLM-13 cells were cultured in CM for 4 hours prior to administration of quizartinib and candidate inhibitors. Inhibitors were identified by literature review of kinases implicated in STAT5 phosphorylation in AML. Cells were harvested following 4 hours of culture with inhibitor, fixed (BD Phosflow Fix Buffer I) and permeabilized (BD Phosflow Perm Buffer III). Cells were analyzed for fluorescence intensity on a BD LSRFortessa cell analyzer.
Patel A.B., Pomicter A.D., Yan D., Eiring A.M., Antelope O., Schumacher J.A., Kelley T.W., Tantravahi S.K., Kovacsovics T.J., Shami P.J., O’Hare T, & Deininger M.W. (2020). Dasatinib overcomes stroma-based resistance to the FLT3 inhibitor quizartinib using multiple mechanisms. Leukemia, 34(11), 2981-2991.
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2

Quantifying STAT5 Phosphorylation in AML

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was performed with Alexa Fluor 647 mouse anti-pSTAT5Y694 (clone 47, BD Biosciences). MOLM-13 cells were cultured in CM for 4 hours prior to administration of quizartinib and candidate inhibitors. Inhibitors were identified by literature review of kinases implicated in STAT5 phosphorylation in AML. Cells were harvested following 4 hours of culture with inhibitor, fixed (BD Phosflow Fix Buffer I) and permeabilized (BD Phosflow Perm Buffer III). Cells were analyzed for fluorescence intensity on a BD LSRFortessa cell analyzer.
Patel A.B., Pomicter A.D., Yan D., Eiring A.M., Antelope O., Schumacher J.A., Kelley T.W., Tantravahi S.K., Kovacsovics T.J., Shami P.J., O’Hare T, & Deininger M.W. (2020). Dasatinib overcomes stroma-based resistance to the FLT3 inhibitor quizartinib using multiple mechanisms. Leukemia, 34(11), 2981-2991.
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