Phage 6tag puro vector

Specific shRNAs against LINC01503, SFPQ, FOSL1, ALX3, and AR were designed by the BLOCK-iT^™ RNAi Designer (http://rnaidesigner.thermofisher.com) and then synthesized according to sequences shown in Supplementary Table 5. All shRNA sequences were cloned into the pLKO.1 vector (Addgene, Cambridge, MA, USA). The full-length sequences of LINC01503, SFPQ, FOSL1, and AR were amplified and cloned into the pHAGE-6tag-puro vector (Addgene). The wild type and mutant LINC01503 and FOSL1 promoters were cloned into the pGL3 vector (Addgene). Specific primers were listed in Supplementary Table 5.
All of the plasmids were confirmed by DNA sequencing, transiently transfected into NPC cells with Lipofectamine 2000/3000 reagents (Invitrogen), and the transfected cells were then harvested for assays 48 h later. The shLINC01503 (sh1503) or its scramble control (shCtrl) plasmids were co-transfected into HEK293T cells with the lentivirus packaging plasmids pMD2G and psPAX2 (Addgene). After 48 h incubated, the lentivirus supernatant was collected and used to infect HK1 and SUNE1 cells, and stably transfected cells were selected and maintained using puromycin.

Specific shRNA sequences against LINC00173, PA24G and SDF4 were designed using the BLOCK‐iT™ RNAi Designer (http://rnaidesigner.thermofisher.com), synthesized by Tsingke Biotechnology Co., Ltd (Beijing, China) and then cloned into the pLKO.1 plasmid (Addgene, Cambridge, MA, USA). The full‐length sequences of LINC00173 transcript (Ensemble Transcript ID: ENST00000470091; 435 nt) and the CDS sequences of RAB1B and GAPDH were amplified using Q5® Hot Start High‐Fidelity 2X Master Mix (New England Biolabs, Inc., Ipswich, MA, USA) and cloned into the pHAGE‐6tag‐puro vector (Addgene). All primers are listed in Table S1.