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Anti actin clone ac 40 primary antibody

Manufactured by Merck Group

Anti-actin (clone AC-40) primary antibody is a laboratory reagent used to detect the actin protein in various biological samples. It is a mouse monoclonal antibody that specifically binds to actin, a ubiquitous cytoskeletal protein found in all eukaryotic cells.

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2 protocols using anti actin clone ac 40 primary antibody

1

DDT and Apoptosis Pathway Analysis

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We purchased p,p’-DDT (1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane; product number 31041-100MG) from Sigma-Aldrich (www.sigmaaldrich.com), and propidium iodide from Abcam (www.abcam.cz: ab14085). For the western blot analysis, we used the following primary and secondary antibodies: anti-cleaved caspase-6 (#9761), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505) anti-cleaved and total PARP (#9542), anti-GRP78 (#3177), and anti-CHOP (#2895) from Cell Signaling Technology (www.cellsignal.com). We purchased anti-actin (clone AC-40) primary antibody from Sigma-Aldrich (www.sigmaaldrich.com: A4700), and corresponding horseradish peroxidase-conjugated secondary antibodies from Proteintech (www.ptglab.com: SA00001-2, and SA00001-1).
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2

Evaluating Apoptosis Signaling Pathways

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We purchased p,p'-DDT (1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane; product number 31041-100MG) from Sigma-Aldrich (www.sigmaaldrich.com), and propidium iodide from Abcam (www.abcam.cz; ab14085). For the western blot analysis, we used the following primary and secondary antibodies: anti-cleaved caspase-6 (#9761), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505) anti-cleaved and total PARP (#9542), anti-GRP78 (#3177), and anti-CHOP (#2895) from Cell Signaling Technology (www.cellsignal.com). We purchased anti-actin (clone AC-40) primary antibody from from Sigma-Aldrich.
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