After an overnight fast, blood samples were collected into ethylenediaminetetra-acetic acid (EDTA)-coated tubes. EDTA plasma and serum samples were aliquoted and stored at −80°C according to standard procedures until further analysis. Plasma Aβ40, Aβ42, t-tau and NfL were measured on a single molecule array (Simoa) platform (Quanterix Corp, MA, USA) following the manufacturer’s instructions (Wayen Biotechnologies Inc., Shanghai, China).30 (link) We used the Human Neurology 3-Plex A assay to measure plasma Aβ and t-tau and the NF-light® Advantage Kit for plasma NfL. We used ‘Vascular Injury Panel 2 (human)’ commercial kit from Meso Scale Discovery to quantify serum cytokine concentrations. The concentrations of serum-soluble intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were assayed following the manufacturer’s instructions. The quality control procedure was described elsewhere.31 (link)
Nf light advantage kit
Manufactured by Mesoscale
Sourced in China, United States
The NF-light® Advantage Kit is a laboratory equipment product designed for the detection and analysis of biomolecules. It provides the core function of separating and visualizing proteins, nucleic acids, and other macromolecules using gel electrophoresis technology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nf light advantage kit
1
Plasma Biomarker Quantification for Neurological Assessment
2
Genetic and Biomarker Profiling for Alzheimer's
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Multiple‐PCR amplification analysis of genomic DNA was performed for 5508 participants to detect 67 single nucleotide polymorphism (SNP) risk loci (eg, APOE, translocase of outer mitochondrial membrane 40 [TOMM40], and KIdney and BRAin expressed protein [KIBRA] genes) and exon region of 20 genes that were potentially associated with AD and dementia.35 In a subsample from the same villages as the MRI subsample (n = 1452), plasma total‐tau, amyloid beta 40 (Aβ40), Aβ42, and neurofilament light chain (NfL) levels were measured on a single molecule array (SIMOA) platform (Quanterix Corp, MA, USA) with Human Neurology 3‐Plex A assay (N3PA) and NF‐light advantage Kit, following the manufacturers’ instructions.36 In addition, commercial kits (Meso Scale Discovery [MSD] V‐PLEX Assays) were used to measure serum cytokines and vascular injury biomarkers (n = 1872): interferon gamma (IFN‐γ), interleukin‐6 (IL‐6), interleukin‐8 (IL‐8), interleukin‐10 (IL‐10), tumor necrosis factor alpha (TNF‐α), interleukin‐17A (IL‐17A), eotaxin‐3, monocyte chemotactic protein‐1 (MCP‐1), intercellular cell adhesion molecule‐1 (ICAM‐1), and vascular cell adhesion molecule‐1 (VCAM‐1).
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