Imaging of the organ‐chip was performed at 20× on a Zeiss 710 ELYRA PS.1 confocal microscope using an EC PlanNeofluar10×/0.3 M27 objective (Zeiss, Oberkochen, Germany). Confocal z‐sections were made throughout the cell depth (approximately 20 sections) using 5 µm step size with an image format of 2048 × 2048 yielding a pixel size of 0.415 µm × 0.415 µm (image size ≈850 µm × 850 µm).
Ec planneofluar10 0.3 m27 objective
Manufactured by Zeiss
Sourced in Germany
The EC PlanNeofluar10×/0.3 M27 objective is a high-quality optical component designed for use in advanced microscopy applications. It features a 10x magnification and a numerical aperture of 0.3, enabling clear and detailed imaging. The M27 thread mount ensures compatibility with a wide range of microscope systems.
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Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Gaasp detector, by Zeiss (1 mentions)
Lsm 880 airyscan, by Zeiss (1 mentions)
Airyscan module, by Zeiss (1 mentions)
Alexa fluor 647 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
710 elyra ps 1, by Zeiss (1 mentions)
Cd326, by Thermo Fisher Scientific (1 mentions)
3 protocols using ec planneofluar10 0.3 m27 objective
1
Confocal Imaging of Organ-on-Chip
2
Confocal Imaging with Zeiss LSM 880 Airyscan
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For confocal imaging, we used the LSM 880 Airyscan (Carl Zeiss, Jena, Germany) scanning laser confocal microscope equipped with an AiryScan module and GaAsP detector (Carl Zeiss, Jena, Germany). Z-stack images (maximum projection) were obtained with an EC Plan-Neofluar 10×/0.3 M27 objective (Carl Zeiss, Obercochen, Germany). Additionally, 405, 488 and 561 nm laser lines were used to detect NucBlueTM Fixed Cell Reagent, calcein and ethidium homodimer-1, respectively.
3
Comprehensive Cell Imaging Protocol
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Cells in both channels on chips were stained for DAPI (1 ug/mL) and Alexa Fluor-647 conjugated Phalloidin (both ThermoFisher Scientific), as well as cancer cell-specific marker EpCAM (CD326, Catalogue #53-8326-42, Thermofisher Scientific). Imaging was performed at 20× on a Zeiss 710 ELYRA PS.1 confocal microscope using an EC Plan-Neofluar10×/0.3 M27 objective (Zeiss, Oberkochen, Germany). Confocal z-sections were made throughout the cell depth (approximately 20 sections) using 5 μm step size with an image format of 2048 × 2048 yielding a pixel size of 0.415 × 0.415 μm (image size approximately 850 × 850 μm).
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