All chemicals were of
reagent grade, made
up in Nanopure water, and passed through 0.2 μm pore size filters.
Yeast extract and tryptone were from Becton Dickinson and Company
(MD, USA). NaCl, sucrose, Tris base, KCl, Na2HPO4, NaH2PO4, EDTA, acetic acid, methanol, and
nickel sulfate were from J.T. Baker (NJ, USA). Ampicillin was from
Fisher (NJ, USA). IPTG was from OmniPur (NJ, USA). Kanamycin, chloramphenicol,
lysozyme, and sodium deoxycholate were from Sigma (MO, USA). Benzonase
was from Novagen (NJ, USA). Imidazole was from EMD (NJ, USA). Coomassie
brilliant blue R-250 was from Bio-Rad Laboratories (CA, USA). Glucose
was from Mallinckrodt (KY, USA). Nonidet P40 substitute was from USB
(OH, USA). ATP and DEAE Sepharose Fast Flow (FF), Q-Sepharose, HisTrap
FF, 16/10 heparin FF, Mono Q, and Mono S 5/50 GL columns were from
GE Healthcare Life Sciences (NJ, USA). Phosphoenol pyruvate (PEP),
nicotinamide adenine dinucleotide (NADH), pyruvate kinase (PK), lactate
dehydrogenase (LDH), and ssDNA-cellulose resin were from Sigma. Phosphocellulose
(P11) was from Whatman. Bio-Gel HTP hydroxyapatite was from Bio-Rad.
Dithiothreitol (DTT) was from Acros Organics. BSA and HindIII were purchased from New England Biolabs. Wheat germ topoisomerase
I (WGT) was from Promega.
reagent grade, made
up in Nanopure water, and passed through 0.2 μm pore size filters.
Yeast extract and tryptone were from Becton Dickinson and Company
(MD, USA). NaCl, sucrose, Tris base, KCl, Na2HPO4, NaH2PO4, EDTA, acetic acid, methanol, and
nickel sulfate were from J.T. Baker (NJ, USA). Ampicillin was from
Fisher (NJ, USA). IPTG was from OmniPur (NJ, USA). Kanamycin, chloramphenicol,
lysozyme, and sodium deoxycholate were from Sigma (MO, USA). Benzonase
was from Novagen (NJ, USA). Imidazole was from EMD (NJ, USA). Coomassie
brilliant blue R-250 was from Bio-Rad Laboratories (CA, USA). Glucose
was from Mallinckrodt (KY, USA). Nonidet P40 substitute was from USB
(OH, USA). ATP and DEAE Sepharose Fast Flow (FF), Q-Sepharose, HisTrap
FF, 16/10 heparin FF, Mono Q, and Mono S 5/50 GL columns were from
GE Healthcare Life Sciences (NJ, USA). Phosphoenol pyruvate (PEP),
nicotinamide adenine dinucleotide (NADH), pyruvate kinase (PK), lactate
dehydrogenase (LDH), and ssDNA-cellulose resin were from Sigma. Phosphocellulose
(P11) was from Whatman. Bio-Gel HTP hydroxyapatite was from Bio-Rad.
Dithiothreitol (DTT) was from Acros Organics. BSA and HindIII were purchased from New England Biolabs. Wheat germ topoisomerase
I (WGT) was from Promega.