AAV vectors were generated by the University of Pennsylvania Vector Core facility by triple transfection of subconfluent HEK293 cells using three plasmids: an AAV trans-plasmid encoding AAV2 capsid, an adenovirus helper plasmid pΔF6, and an AAV cis shuttle plasmid expressing eGFP driven by a CMV promoter (pENN.AAV.CMV.PI.eGFP.WPRE.bGH). The culture medium was collected, concentrated by tangential flow filtration and purified by iodixanol gradient ultracentrifugation as previously described [68 (link)]. pGEM-T vector plasmid containing the mouse UCHL1 cDNA ORF clone was purchased from Sino Biological (cat: MG50690-G, Wayne, PA, USA), and the UCHL1 CDS was subcloned into a AAV plasmid with CBA promoter [69 (link)], to generate pAAV.CBA.UCHL1-IRES-eGFP.WPRE plasmid that was packaged into AAV2 virus particles by the University of Pennsylvania Vector Core facility as described above.
Uchl1 cdna orf clone
Manufactured by Sino Biological
Sourced in United States
UCHL1 cDNA ORF clone is a laboratory tool that contains the full-length coding sequence of the UCHL1 gene. UCHL1, also known as ubiquitin carboxyl-terminal hydrolase L1, is an enzyme involved in the regulation of the ubiquitin-proteasome system. This clone can be used for various research applications, such as gene expression studies and protein production.
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2 protocols using uchl1 cdna orf clone
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AAV Vector Production for Protein Expression
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AAV Vector Production for Protein Expression
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AAV vectors were generated by the University of Pennsylvania Vector Core facility by triple transfection of subconfluent HEK293 cells using three plasmids: an AAV trans-plasmid encoding AAV2 capsid, an adenovirus helper plasmid pΔF6, and an AAV cis shuttle plasmid expressing eGFP driven by a CMV promoter (pENN.AAV.CMV.PI.eGFP.WPRE.bGH). The culture medium was collected, concentrated by tangential flow filtration and purified by iodixanol gradient ultracentrifugation as previously described [68 (link)]. pGEM-T vector plasmid containing the mouse UCHL1 cDNA ORF clone was purchased from Sino Biological (cat: MG50690-G, Wayne, PA, USA), and the UCHL1 CDS was subcloned into a AAV plasmid with CBA promoter [69 (link)], to generate pAAV.CBA.UCHL1-IRES-eGFP.WPRE plasmid that was packaged into AAV2 virus particles by the University of Pennsylvania Vector Core facility as described above.
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