Rat anti ly6g ab

Manufactured by BD

Rat anti-Ly6G Ab is a monoclonal antibody that binds to the Ly6G antigen, which is primarily expressed on the surface of neutrophils. This antibody can be used to identify and detect neutrophils in various biological samples.

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Lab products found in correlation

Rabbit anti cd11b ab, by Abcam (2 mentions) Envision system horseradish peroxidase kit, by Agilent Technologies (1 mentions) Rabbit anti rxfp1 ab, by Santa Cruz Biotechnology (1 mentions) Rat anti cd68 ab, by Bio-Rad (1 mentions) Rabbit anti ho 1 ab, by Enzo Life Sciences (1 mentions)

Close spelling variants of this product

Rat anti-Ly6G Anti-Ly6G

2 protocols using rat anti ly6g ab

Immunohistochemical Assessment of RXFP1 Expression

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The expression of RXFP1 (liver and heart) was examined using rabbit anti-RXFP1 Ab (Santa Cruz Biotechnology, Santa Cruz, CA). Immunostaining signals were visualized with a labeled polymer in the EnVision+ system horseradish-peroxidase kit (Dako, Carpinteria, CA). Liver infiltrating neutrophils and macrophages were detected with rat anti-Ly6G Ab (BD Biosciences, San Jose, CA) and rabbit anti-CD11b Ab (Abcam, Cambridge, MA), respectively. Signals were visualized with secondary Alexa Fluor 488 IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (×400).

Kageyama S., Nakamura K., Ke B., Busuttil R.W, & Kupiec-Weglinski J.W. (2018). Serelaxin Induces Notch1 Signaling and Alleviates Hepatocellular Damage in Orthotopic Liver Transplantation. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(7), 1755-1763.

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Liver Immune Cell Profiling

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Mouse livers were stained with rabbit anti-CD11b Ab (Abcam, Cambridge, MA), rat anti-Ly6G Ab (BD Biosciences, San Jose, CA), rabbit anti-4-hydroxynonenal Ab (Abcam), rabbit anti-HO-1 Ab (Enzo Life Sciences, Farmingdale, NY) and rat anti-CD68 Ab (Bio Rad, Hercules, CA). Human livers were stained with rabbit anti-HO-1 Ab (Enzo Life Sciences) and mouse anti-CD68 Ab (BD Biosciences). Signals were visualized with secondary Alexa Fluor Abs. Liver-infiltrating CD11b+ and Ly6G+ cells were scored semi-quantitatively by blindly counting positive cells in 10 HPF/section (x400).

Kageyama S., Hirao H., Nakamura K., Ke B., Zhang M., Ito T., Aziz A., Oncel D., Kaldas F.M., Busuttil R.W., Sosa R.A., Reed E.F., Araujo J.A, & Kupiec-Weglinski J.W. (2018). Recipient HO-1 Inducibility is Essential for Post-Transplant Hepatic HO-1 Expression and Graft Protection: From Bench-to-Bedside. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(2), 356-367.

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