Anti cd3 percp vio700

UltraComp eBeads^TM Compensation Beads (Thermofisher) were used to optimize fluorescence compensation settings for multi-color flow cytometric analysis at a Symphony flow cytometer. UltraComp eBeads^TM were stained with the following fluorochrome-labeled anti-human antibodies: anti-CD8–BUV805 (1:200, clone SK1), anti-CD4–BUV496 (1:50, clone SK3), anti-CD86–BUV737 (1:50, clone 2331 FUN-1), anti-CD141–BUV615-P (1:50, clone 1A4), anti-CD56–BUV563 (1:50, clone NCAM 16.2), anti-CD16–BUV395 (1:50, clone 3G8), anti-CD123–BB660-P (1:50, clone 7G3), anti-CD80–BB630 (1:50,clone L307.4), anti-CD21–BV785 (1:50, clone B-ly4), anti-CD27–BV750-P (1:40,clone L128), anti-BAFF-R–BV650 (1:50, clone 11C1), anti-CD94–BV605 (1:50, clone HP-3D9), anti-CD40–APC-R700 (1:50, clone 5C3) (all BD bioscience); anti-CD3–PerCP-Vio700 (1:50, clone REA613) (Miltenyi Biotec); anti-CD57–FITC (1:100, clone TB01), anti-CD14–PE-Cy5.5 (1:200, clone TuK4), fixable viability dye eFluor780 (1:1000) (all eBioscience); anti-CD24–BV711 (1:50, clone ML5), anti-CD19–BV510 (1:25, clone HIB19), anti-HLA-DR–BV570 (1:40, clone L243), anti-IgM–BV421 (1:100, clone MHM-88), anti-CD11c–APC (1:40, clone 3.9), anti-CD38–PE/Dazzle 594 (1:100, clone HB-7), anti-CD10–PE-Cy5 (1:50, clone HI10a), and anti-IgD–PE-Cy7 (1:100, clone IA6-2) (all BioLegend).