Jxfstprp cl

Manufactured by Jingxin

Sourced in China

The JXFSTPRP-CL is a lab equipment product designed for scientific applications. It serves as a core functional component for various laboratory processes. The detailed specifications and intended use of this product are not available at this time.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Mak030, by Merck Group (1 mentions) Mak022, by Merck Group (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Af2006, by Proteintech (1 mentions) Phospho nfκb, by Affinity Biosciences (1 mentions) Bca assay kit, by Yeasen (1 mentions) Nlrp3, by Abcam (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions) Poroshell ec c18 column, by Agilent Technologies (1 mentions) Agilent 1200 series hplc, by Agilent Technologies (1 mentions) Interleukin 6 (il 6), by Cloud-Clone (1 mentions) Varioskan lux, by Thermo Fisher Scientific (1 mentions) Il 10, by Cloud-Clone (1 mentions) Tnf α, by Cloud-Clone (1 mentions) Il 1β, by Cloud-Clone (1 mentions) Inductively coupled plasma mass spectrometry, by Thermo Fisher Scientific (1 mentions)

7 protocols using jxfstprp cl

Characterization of Ground TCP Particles

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The
as-received TCP powder was ground using a freezing ball mill (JXFSTPRP-CL,
Jingxin, Shanghai, China; frequency = 60 Hz) for 30 min at −10
°C; the ground TCP particles were denoted as TCP-G. Both TCP
and TCP-G particles were observed using a laser confocal microscope
(LSM700, Carl Zeiss, Germany). The size and distribution of both TCP
and TCP-G particles were determined using dynamic light scattering
(DLS). The TCP and TCP-G particles were dispersed in anhydrous ethanol
at 0.1 mg/mL. Then, the particle size was measured using a Brookhaven
Zeta BI-PALS zeta-sizer at 25 °C. Alkalinity of the TCP/TCP-G
powder was measured as described above. The Ca²⁺/PO₄^3– concentrations of the supernatant were
examined using a microplate reader (SynergyH1, BioTek, USA) using
a calcium colorimetric assay kit (MAK022, Sigma-Aldrich, USA) and
a phosphate colorimetric assay kit (MAK030, Sigma-Aldrich, USA), respectively.

Huang Q., Liang Z., Li J., Bai Y., He J, & Lin Z. (2021). Size Dependence of Particulate Calcium Phosphate Fillers in Dental Resin Composites. ACS Omega, 6(50), 35057-35066.

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Western Blot Analysis of Lung Tissues

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The Western blot was performed as in a previous study (22 (link)). The lung tissues and cells were collected and added proteinase inhibitor (Vazyme), phosphatase inhibitor (Vazyme), and radio immunoprecipitation assay (RIPA) (Beyotime). Then the mixes were split by using the grinding machine (Jingxin, Shanghai; JXFSTPRP-CL) and centrifuged at 12,000 rpm for 15 min to collect the supernatant. The concentration of supernatant protein was detected by using BCA assay kit (Yeasen). After separation and transfer, the corresponding proteins were measured by using the following antibodies: Nlrp3 (Abcam; ab263899), Phospho-NF-κB (Affinity; AF2006), and Actin (Proteintech; 66009-1-Ig).

Chen J., Huang Y., Bian X, & He Y. (2022). Berberine Ameliorates Inflammation in Acute Lung Injury via NF-κB/Nlrp3 Signaling Pathway. Frontiers in Nutrition, 9, 851255.

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Microwave-Assisted Sulforaphane Extraction

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The microwave-treated samples were placed in the automatic grinder (JXFSTPRP-CL, Shanghai Jingxin Industrial Development Co., LTD, China) at 30, 40, 50, 60 and 70 Hz for grinding 5, 10, 20, 30 and 40 s respectively, then were degreased. Afterwards, 4 mL distilled water and 4 mL ethyl acetate were added to extract sulforaphane, then analyzed by HPLC.

Liu Y., Zhang D., Li X., Xiao J, & Guo L. (2022). Enhancement of ultrasound-assisted extraction of sulforaphane from broccoli seeds via the application of microwave pretreatment. Ultrasonics Sonochemistry, 87, 106061.

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Quantitative Hair Analysis by HPLC-MS

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The analysis was performed on an Agilent-1200 series HPLC system coupled to an Agilent G6130A single quadrupole MS instrument (Agilent Technologies, Santa Clara, CA, USA). The separation of the extracted compounds was carried out on an Agilent Poroshell EC-C18 column (2.1 mm × 100.0 mm, 1.9 µm) at 40 °C. The mobile phase was methanol (solvent A) and 0.1% formic acid aqueous solution (solvent B), respectively. Gradient elution was performed at 0.3 mL/min with a total run time of 15.0 min: 20% B held for 1 min, 1–4 min, 20%B→5%B and held for 4 min; 8–8.1 min, 5%B→20%B; and finally held for 6.9 min.
The electrospray ionization (ESI) was set at positive ion mode. Source conditions were set as follows: drying gas (N₂) flow 10 L/min; drying gas temperature 350 °C; capillary voltage 3.0 kV. The instrument was operated in the scan mode in the range from 50–750 (m/z) for qualitative analysis and selected ion monitoring (SIM) mode for quantitative analysis with the parameters described in Table 1.
High-efficiency hair grinder (JXFSTPRP-CL; Jingxin, Shanghai, China) was used for hair samples preparation.

Meng L., Dai Y., Chen C, & Zhang J. (2020). Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS. Forensic Sciences Research, 6(3), 273-280.

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Quantifying Neuroimmune Biomarkers in Brain Tissue

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The concentrations of 5-hydroxytryptamine (5-HT), dopamine (DA) (Elabscience Biotechnology, China), brain-derived neurotrophic factor (BDNF), tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-10 (Cloud-Clone Corp., China) were detected by ELISA. A commercial kit was used to measure each cytokine level in plasma or the supernatants of brain tissues according to the manufacturer's instructions. Briefly, the brain tissues were rinsed with ice-cold phosphate-buffered saline (PBS; 0.01 M, pH = 7.4) to remove any excess blood. They were then weighed, minced, and homogenized in PBS with an automatic sample freeze-grinding instrument (JXFSTPRP-CL, Shanghai Jingxin Industrial Development Co., Ltd, China) at 0 °C. The homogenates were then centrifuged for 5 min at 5000g to obtain the supernatant. The absorbance was read at 450 nm using a spectrophotometer (Varioskan LUX, Thermo Fisher Scientific, USA), and the brain sample concentrations were subsequently calculated using the equation generated from a standard curve.

Guo L., Xiao P., Zhang X., Yang Y., Yang M., Wang T., Lu H., Tian H., Wang H, & Liu J. (2021). Inulin ameliorates schizophrenia via modulation of the gut microbiota and anti-inflammation in mice. Food & function, 12(3).

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Collagen Extraction and Chondrocyte Matrix

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The rats were anesthetized with 5% chloral hydrate (2 mL) and killed by breaking their necks. The tail skin was cut open, and the tail tendon was removed and cleaned with saline. The tail tendon was cut into pieces and placed in 150 mL of 0.5% acetic acid. The tendon was then shaken and dissolved for 48 h at 4 °C. It was then centrifuged at 12,000 rpm and the supernatant was added to a 10% NaCl solution to precipitate the collagen. An appropriate volume of HCL (0.2 mM) was added to dissolve the supernatant, and the supernatant was stored at 4 °C for later use.
The costal cartilage of the rats (aged 2–3 weeks) was removed, cut into pieces and then grinded with a tissue grinder (70 Hz, 2 min, 3 times) (JXFSTPRP-CL, Shanghai Jing Xin, Shanghai, China). Tissue homogenate was obtained, and 4 mL of medium was added for dilution. Then the filtrate was collected through a 0.22-μm filter to obtain the chondrocyte extracellular matrix. 50 μL of cartilage extracellular matrix was added to 700 μL of hydrogel, and 250 μL of 1 × 10⁵ CESCs were added. After mixing, the gel was formed over 5–10 min at 37 °C. This constructed the hydrogel loaded with CESCs that were modified by the cartilage extracellular matrix.

Luo L., Gong J., Wang Z., Liu Y., Cao J., Qin J., Zuo R., Zhang H., Wang S., Zhao P., Yang D., Zhang M., Wang Y., Zhang J., Zhou Y., Li C., Ni B., Tian Z, & Liu M. (2021). Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. Bioactive Materials, 15, 29-43.

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Biogenic Tellurium Nanoparticle Synthesis

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A. pittii D120 was inoculated into MM containing sodium tellurite at a cell concentration of 1 × 10⁶ CFU mL⁻¹ and incubated at 37 °C with shaking at 150 rpm under dark. The purification of BioTe was adopted from previous research with minor modifications [9 (link)]. After cultivation and synthesis for 72 h, cells and synthesized BioTe were collected by centrifugation (10,000× g, 10 min). The precipitate was washed three times and resuspended in deionized water. The resuspended samples were ground at 60 Hz for 6 min by using a freezing grinder JXFSTPRP-CL (Jingxin, Shanghai, China). The BioTe were separated from cell debris by centrifugation at 12,000 × g for 10 min. Finally, the supernatant containing BioTe was filtered through a 0.22 μm filter for sterilization. BioTe solution was stored at 4 °C before use. The concentration of BioTe solution was determined by inductively coupled plasma mass spectrometry (Thermo Fisher Scientific, Waltham, MA, USA).

Tang A., Ren Q., Wu Y., Wu C, & Cheng Y. (2022). Investigation into the Antibacterial Mechanism of Biogenic Tellurium Nanoparticles and Precursor Tellurite. International Journal of Molecular Sciences, 23(19), 11697.

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