Log In Sign up
The largest database of trusted experimental protocols

The HT-29 is a human colorectal adenocarcinoma cell line. It is a commonly used in vitro model for the study of colorectal cancer.

Automatically generated - may contain errors

Lab products found in correlation

Hct116, by Shanghai Institute of Biochemistry and Cell Biology (5 mentions) Hek293t, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Sw480, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Sw620, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Sw620, by Thermo Fisher Scientific (1 mentions) Caco 2, by Thermo Fisher Scientific (1 mentions) Sw480, by Thermo Fisher Scientific (1 mentions) Ht 29, by Thermo Fisher Scientific (1 mentions) Hct116, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Caco 2, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions) Dual luciferase detection kit, by Promega (1 mentions) Ripa lysate, by Thermo Fisher Scientific (1 mentions) Primary antibodies against mtor, by Abcam (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Mtt kit, by Merck Group (1 mentions) Transwell insert, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Gapdh, by Abcam (1 mentions)

7 protocols using ht 29

1

Colorectal Cancer Patient Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human specimens and cell lines All samples were collected from CRC patients in the First A liated Hospital of Nanjing Medical University.
Their blood samples were also collected before resection on the day of surgery. Besides, a total of 50 cases of human blood samples were obtained from healthy donors. The ethics permission number is 2019-SRFA-131.
Cell lines (HCT 116, HT-29, and HEK293T) were purchased from the Shanghai Institute of Cell Biology and cultured in McCoy'5A and RPMI-1640 medium ( HyClone, Logan) supplemented with 10% FBS.
Yang P., Zhang D., Wang T., Jin C., Peng C., Ji J., Tan Y., Zhou J., Wang L., & Sun Y. (2022). High WEE2-AS1 expression in CAF-derived small extracellular vesicles inhibits the Hippo pathway to facilitate colorectal cancer progression via promoting degradation of MOB1A.
+ Open protocol
+ Expand
2

Transfection of Colorectal Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human colorectal cancer cells HCT116 and HT-29 were obtained from the Shanghai Institute of Cell Biology (Shanghai, China) and were maintained in DMEM supplemented with 10% FBS plus 2 mM glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin. Transient transfection of HCT116 cells were transfected with 2 μg of HIBCH plasmid or shHIBCH (sh1: CCATACAGAGTCTAAGATT; sh2: GGTTACTTCCTTGCATTAA) using the Lipofectamine® 3000 (Invitrogen, Carlsbad, CA, USA) reagents by following the manufacturer’s instructions.
Shan Y., Gao Y., Jin W., Fan M., Wang Y., Gu Y., Shan C., Sun L., Li X., Yu B., Luo Q, & Xu Q. (2019). Targeting HIBCH to reprogram valine metabolism for the treatment of colorectal cancer. Cell Death & Disease, 10(8), 618.
+ Open protocol
+ Expand
3

Colon Cancer Tissue Collection and Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
We collected tumor samples and distant normal mucous tissues (>5 cm from the margin of the tumor) from 21 patients with colon cancer who received surgical resection at our hospital from January 2015 to December 2016. No patient had received neoadjuvant chemotherapy or chemotherapy prior to surgery, and all tissue samples were pathologically confirmed. Tumor-node-metastasis staging of the patients was according to the 2015 National Comprehensive Cancer Network clinical practice guidelines. Informed consent was obtained from each patient from the archives of the Central Hospital of Panyu District Hospital and with approval from the institutional ethics committee (Guangzhou, China).
Cell culture FIHC, HCT-116, HT-29, and SW480 cell lines were purchased from the Shanghai Institute of Cell Biology (Shanghai, China). The cell lines were cultured in Dulbecco's Modified Eagle's Medium (DMEM)/Nutrient Mixture F-12 Ham containing 10% fetal bovine serum, and maintained in a humidified incubator at 37°C with 5% CO 2 . The cells in logarithmic growth with 95% viability were subjected to further experiment.
He J.H., Li Y.G., Han Z.P., Zhou J.B., Chen W.M., Lv Y.B., He M.L., Zuo J.D, & Zheng L. (2018). The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback Circuit Affects the Proliferation, Migration, and Invasion of Colon Cancer SW480 Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(4).
+ Open protocol
+ Expand
4

CRC Patient Blood Sampling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All samples were collected from CRC patients in the First Affiliated Hospital of Nanjing Medical University. Their blood samples were also collected before resection on the day of surgery. Besides, a total of 50 cases of human blood samples were obtained from healthy donors. All subjects signed informed consent. The ethics permission number is 2019-SRFA-131.
Cell lines (HCT 116, HT-29, and HEK293T) were purchased from the Shanghai Institute of Cell Biology and cultured in McCoy’5A and RPMI-1640 medium (HyClone, Logan) supplemented with 10% FBS.
Yang P., Zhang D., Wang T., Ji J., Jin C., Peng C., Tan Y., Zhou J., Wang L., Feng Y, & Sun Y. (2022). CAF-derived exosomal WEE2-AS1 facilitates colorectal cancer progression via promoting degradation of MOB1A to inhibit the Hippo pathway. Cell Death & Disease, 13(9), 796.
+ Open protocol
+ Expand
5

Establishing Oxaliplatin-Resistant Colorectal Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Six human colorectal cancer cell lines HCT116, SW480, HT29, SW620, Caco2 and LOVO were obtained from the Shanghai Institute of Cell Biology (Shanghai, China).The related oxaliplatin-resistant cell lines SW480-OxR and HCT116-OxR were generated by continuous exposure to increasing concentrations of oxaliplatin for a 10-month period as described previously 23 (link). We performed cytotoxicity testing to confirm that chemoresistance could be stable for about 4 weeks without oxaliplatin exposure. The oxaliplatin-resistant cell lines were used at no higher than 15 passages from creation. All cells were cultured in the appropriate medium (RPMI-1640 for HT29, SW620, SW480 and SW480-OxR cells; DMEM for Caco2, LOVO, HCT116 and HCT116-OxR cells) supplemented with 10% FBS (Gibco, Carlsbad, CA, USA) in a humidified atmosphere with 5% CO2 at 37 °C.
Sun W., Li J., Zhou L., Han J., Liu R., Zhang H., Ning T., Gao Z., Liu B., Chen X, & Ba Y. (2020). The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. Theranostics, 10(5), 1981-1996.
+ Open protocol
+ Expand
6

Cell culture of colorectal cancer lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adult male BALB/c mice and nude mice were purchased from Shanghai Laboratory Animal Research Center (Shanghai, China) and raised under Specific Pathogen Free (SPF) conditions. HCT116 and HT-29 cell lines were purchased from Shanghai Institute of Cell Biology. Cells were cultured with McCoy’5A medium containing 10% fetal bovine serum (FBS) at 37 °C with 5% CO2.
Zhang X., Tang W., Wen H., Wu E., Ding T., Gu J., Lv Z, & Zhan C. (2022). Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer. Pharmaceutics, 14(4), 868.
+ Open protocol
+ Expand
7

Investigating Colon Cancer Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Healthy colonic epithelial cells (HCoEpiC), human colon cancer cell lines HT‐29, SW620, and SW480, and LoVo and Coco‐2 cell lines were purchased from Shanghai Institute of Cell Biology. Fetal bovine serum (FBS) and DMEM/F12 were purchased from Hyclone; Cytiva. TRIzol®, Lipofectamine® 2000, RIPA lysate, and BCA protein assay kit were purchased from Invitrogen; Thermo Fisher Scientific, Inc. Pcmv plasmid, miR‐96‐5p mimics/inhibitor, and irrelevant nucleic acid sequence were purchased from Shanghai GenePharma Co., Ltd., and Annexin‐V/PI Apoptosis kit was purchased from CapitalBio Technology, Inc. Matrigel was purchased from BD Biosciences, the MTT kit was purchased from Sigma‐Aldrich; Merck KGaA, the Transwell inserts was purchased from MilliporeSigma and the dual‐luciferase detection kit was purchased from Promega Corporation. Primary antibodies against mTOR, LC 3, LC 3B, Beclin1, P62, and GAPDH were purchased from Abcam. mTOR agonist was purchased from BIOLEBO.
Xu J., Zeng W., Liu T., Wan Z., Yang X., Chen J, & Liu F. (2023). lncRNA TINCR knockdown inhibits colon cancer cells via regulation of autophagy. Food Science & Nutrition, 11(4), 1965-1981.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!