Pcna staining kit

Manufactured by Zymo Research

Sourced in United States

The PCNA staining kit is a laboratory tool used to detect and visualize Proliferating Cell Nuclear Antigen (PCNA) in biological samples. PCNA is a protein involved in DNA replication and repair processes. The kit provides reagents and protocols to perform immunohistochemical or immunocytochemical staining of PCNA, allowing researchers to study cell proliferation in various experimental systems.

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Lab products found in correlation

P nf κb p65 ser536, by Cell Signaling Technology (2 mentions) P stat3 tyr705, by Cell Signaling Technology (2 mentions) Rabbit igg control antibody, by R&D Systems (1 mentions)

2 protocols using pcna staining kit

Immunohistochemical Analysis of Colon Cancer

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Colonic cryosections of Apc^min/+ mice and IL-21 KO-Apc^min/+ mice were stained with p-STAT3 Tyr705, p-NF-κB/p65 Ser536 (both from Cell Signaling) and CD31 (BD Pharmingen). Isotype control-stained sections were prepared under identical conditions replacing the primary antibody with either a rabbit or rat IgG control antibody (R&D Systems). Proliferating cells were evaluated using a PCNA staining kit (ZYMED Laboratories, Carlsbad, CA) according to the manufacturer's instructions. Negative control-stained sections were prepared under identical immunohistochemical conditions omitting the primary antibody.

De Simone V., Ronchetti G., Franzè E., Colantoni A., Ortenzi A., Fantini M.C., Rizzo A., Sica G.S., Sileri P., Rossi P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2015). Interleukin-21 sustains inflammatory signals that contribute to sporadic colon tumorigenesis. Oncotarget, 6(12), 9908-9923.

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Colonic inflammation in Apc^min/+ mice

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Colonic cryosections of Apc^min/+ mice were stained with p-STAT3 Tyr705 and p-NF-κB/p65 Ser536 (both from Cell Signaling). Isotype control-stained sections were prepared under identical conditions replacing the primary antibody with a rabbit IgG control antibody (R&D Systems). Proliferating cells were evaluated using a PCNA Staining Kit (Zymed Laboratories, Carlsbad, CA, USA) according to the manufacturer's instructions. Negative control-stained sections were prepared under identical immunohistochemical conditions omitting the primary antibody.

De Simone V., Franzè E., Ronchetti G., Colantoni A., Fantini M.C., Di Fusco D., Sica G.S., Sileri P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2014). Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth. Oncogene, 34(27), 3493-3503.

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