Bxpc 3

Cells were incubated at 6% CO 2 and at 37 °C in monolayer culture under humidified conditions. Human lung lines NCI-H520 and NCI-H596, pancreatic line BxPC3, breast line BT-20, colon line HCT116, and liver line HEPG2 were sourced from the American Type Culture Collection (ATCC; Manassas, VA). Lung line LUDLU-1 was purchased from the European Collection of Cell Cultures (Salisbury, UK). SMMC-7721, a liver cell line, was sourced from Shanghai Medicilon, Inc. (Shanghai, China). Human lung lines LC-1/ sq and LK-2 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). NCI-H520, NCI-H596, LUDLU-1, SMMC-7721, and LK-2 were cultured in RPMI supplemented with 10% fetal bovine serum (FBS; ATCC). BxPC3 was cultured with RPMI with 10% FBS (ATCC), 10 mM HEPES, and 10 mM sodium pyruvate. HEPG2 and BT-20 were maintained with minimum essential media, 10% FBS (ATCC), nonessential amino acids, and 10 mM sodium pyruvate. Lung line LC-1/ sq was grown in a 1:1 RPMI/Ham's F12 medium supplemented with 10% FBS (ATCC). The HCT116 colon line was cultured with McCoy's 5A media with 10% FBS (ATCC). All cell lines tested mycoplasma negative prior to their experimental use. All cell culture media and supplements, except as noted above, were from Life Technologies (Carlsbad, CA).