Seven human PC cell lines (PC346, LNCap, BPH1, MDAPC1 2a/b, C4-2, PC3 and DU145) were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA). These cells were maintained in DMEM (Invitrogen, Rockville, MD, USA) suppled with 10% fetal bovine serum (FBS; Sigma-Aldrich, Rockville, MD, USA) in a humidified incubator at 37 °C with a 5% CO2 atmosphere. R1881 (Sigma-Aldrich) was applied to cells at a concentration of 1 nmol/l and OH-flutamide (OHF, Sigma-Aldrich) was applied to cells at a concentration of 5 µmol/l.
Pc346
Manufactured by American Type Culture Collection
Sourced in United States
The PC346 is a laboratory centrifuge with a fixed-angle rotor configuration. It is designed to separate and concentrate various biological samples, such as cell cultures, bacterial suspensions, and other liquid samples, based on their density and sedimentation rate. The centrifuge can achieve a maximum speed of 15,000 revolutions per minute (RPM) and a maximum relative centrifugal force (RCF) of 21,500 x g. The PC346 is suitable for a wide range of laboratory applications that require high-speed centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Lncap, by American Type Culture Collection (3 mentions)
Du145, by American Type Culture Collection (2 mentions)
Mdapc1 2a b, by American Type Culture Collection (2 mentions)
R1881, by Merck Group (1 mentions)
Bph 1, by American Type Culture Collection (1 mentions)
Crl 2876, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Crl 2242, by American Type Culture Collection (1 mentions)
Htb 81, by American Type Culture Collection (1 mentions)
Crl 3314, by American Type Culture Collection (1 mentions)
Crl 1740, by American Type Culture Collection (1 mentions)
Crl 1435, by American Type Culture Collection (1 mentions)
3 protocols using pc346
1
Culturing and Treating Human PC Cell Lines
2
Culturing Human Prostate Cancer Cell Lines
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Human PCa cell lines (LNCap and PC346) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA), maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen, Rockville, MD, USA) with 8% fetal bovine serum (FBS; Sigma-Aldrich, Rockville, MD, USA) in a humidified incubator at 37 ℃ with a 5% carbon dioxide (CO2). The PC346 cells express high levels of normal ARs and are very sensitive to androgen treatment. The LNCap cells have abnormal ARs with broad steroid binding specificity and represent CRPC.
3
Culturing Human Prostate Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human PC cell lines (PC346, LNCap, MDAPC1 2a/b, C4-2, PC3, and DU145) were obtained from the American Type Culture Collection (Catalog number CRL-2876, CRL-1740, CRL-2242, CRL-3314, CRL-1435, and HTB-81, respectively, ATCC, Rockville, MD, USA). Human PC cell line (BPH1) was obtained from Accegen Biotechnology (Catalog number ABC-TC454S, Fairfield, NJ, USA). All lines were maintained in DMEM (Invitrogen, Rockville, MD, USA) suppled with 5% fetal bovine serum (FBS; Sigma-Aldrich, Rockville, MD, USA) in a humidified incubator at 37 °C with a 5% CO2 atmosphere.
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