HEK293T cells and RBL-2H3 mast cells (ATCC, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA). For stable transfection of HEK293T cells with TRPV3, 10 μg/mL of blasticidin (Thermo Fisher Scientific, Waltham, MA, USA) was added for antibiotic selection. HEK293T cells were grown at 37°C in a humidified incubator with 10% CO2/20% O2. RBL-2H3 cells were grown at 37°C in a humidified 5% CO2 incubator.
Rbl 2h3 mast cells
Manufactured by American Type Culture Collection
Sourced in United States
RBL-2H3 mast cells are a rat basophilic leukemia cell line. They are used in research to study mast cell function and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Blasticidin, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Hacat keratinocytes, by Addexbio (1 mentions)
Jurkat t cells, by Thermo Fisher Scientific (1 mentions)
Jurkat t cells, by American Type Culture Collection (1 mentions)
Hek293t cells, by Welgene (1 mentions)
Rbl 2h3 cells, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin p s, by GE Healthcare (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
5 protocols using rbl 2h3 mast cells
1
Cell Culture and Stable Transfection
2
Culturing HEK293T and RBL-2H3 Cells
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HEK293T cells and RBL-2H3 mast cells (ATCC, Manassas, VA) were cultured in Dulbecco’s modified Eagle’s medium (Life Technologies, Carlsbad, CA) containing 10% foetal bovine serum and 1% penicillin-streptomycin. For stable transfection of HEK293T cells with TRPV3, 10 μg/mL blasticidin (Life Technologies) was added for antibiotic selection. All cells were grown at 37 °C in a humidified incubator with 10% CO2/20% O2.
3
Culturing RBL-2H3 and HaCaT Cells
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RBL-2H3 mast cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and HaCaT keratinocytes were purchased from AddexBio technologies (San Diego, CA, USA). RBL-2H3 and HaCaT cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; 56 °C for 30 min; Gibco, Grand Island, NY, USA) and 1% 100 unit/mL penicillin–streptomycin (Gibco, Grand Island, NY, USA). The cells were grown at 37 °C in a 5% CO2 atmosphere incubator (Sanyo, Osaka, Japan).
4
Culturing Rat Basophilic Leukemia Cells
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Rat basophilic leukemia (RBL)-2H3 mast cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). RBL-2H3 cells were cultured at 37°C in a 5% CO2-humidified incubator, and maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) heat-inactivated fetal bovine serum, 1 mM sodium pyruvate, 2 mM glutamine, 100 U/mL penicillin, and 50 μg/mL streptomycin (Lee et al., 2013 (link)).
5
Cell line maintenance protocols
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Human embryonic kidney 293 T (HEK293T), RBL-2H3 mast cells, and Jurkat T cells were obtained from the American Type Culture Collection (Manassas, VA, USA). HEK293T cells were maintained at 37°C with 10% CO2 in Dulbecco’s modified Eagle’s medium (Welgene, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS; Welgene) and 1% penicillin/streptomycin (P/S; GE Healthcare, Chicago, IL, USA). RBL-2H3 cells were cultured in minimum essential medium (Gibco, Grand Island, NY, USA), whereas Jurkat T cells were grown in RPMI1640 medium (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS and 1% P/S; both cell lines were maintained at 37°C in a 5% CO2 incubator.
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