EMG signals were recorded in bipolar derivations with pairs of Ag/AgCl electrodes (Ambu Neuroline 720 01-K/12; Ambu, Ballerup, Denmark) with 22 mm of center-to-center spacing. Prior to electrode placement the skin was shaved and lightly abraded. A reference electrode was placed on the right tibia. The EMG signals were recorded from a portable EMG amplifier (Biovision EMG-Amp, Germany) stored in a backpack together with a mini-computer. The EMG signals were sampled at 2000 Hz (12 bits per sample), band-pass filtered (second-order, zero lag Butterworth, bandwidth 10–500 Hz). The EMG signals were recorded from the following muscles of the right side (dominant side for 11 out of 12 subjects) according to Barbero et al. (2011 ): tibialis anterior (TA), soleus (SO), gastrocnemius lateralis (GL), gastrocnemius medialis (GM), vastus lateralis (VL), vastus medialis (VM), rectus femoris (RF), biceps femoris (BF), semitendinosus (ST), and gluteus maximus (GX). A uniaxial accelerometer was placed on the right tibia, which measured the vertical acceleration synchronized to the EMG measurements.
Neuroline 720 01 k 12
Manufactured by Ambu
Sourced in Denmark
The Ambu Neuroline 720 01-K/12 is a medical device designed for electrophysiological recordings. It is a pre-gelled, disposable, surface electrode that can be used for various neurological and physiological measurements.
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Lab products found in correlation
5 protocols using neuroline 720 01 k 12
1
Comprehensive Lower Limb EMG Measurement
2
Evaluation of Industrial Prosthetic Control
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To demonstrate the advantage of the proposed approach with respect to current commercial systems, 9 able-bodied subjects participated in an additional experiment, in which the industrial state-of-the-art (SOA) control method was used to perform the target reaching test. The industrial SOA utilized the one-site-one-function approach with two-control sites. Two pairs of electrodes (Ambu® Neuroline 720 01-K/12, Ambu A/S, Denmark) were placed over the flexors and extensors of the wrist, respectively. The signal was recorded in differential mode, resulting in two surface EMG channels, as the electrodes used in most commercial prostheses [14 (link)]. Prosthetic experts selected the sites with the criterion of minimal cross-talk between the two channels. An activation threshold was set for each channel. When the threshold of the channels was exceeded, the corresponding function would be selected (e.g., supination or pronation). When the two thresholds were simultaneously exceeded, a mode switch would take place (e.g. from rotation mode to open/close mode). Individual thresholds of the two channels were chosen through the standard procedure in prosthetic fitting, such that occurrences of un-intended mode switches were minimal while intended activation commands could be easily articulated. The same algorithm was used in a recent online study [15 (link)].
3
Surface EMG Recording of Forearm Muscles
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Surface EMG were recorded using six pairs of electrodes (Ambu® Neuroline 720 01-K/12, Ambu A/S, Denmark) mounted on the dominant forearm. The electrode pairs were equally spaced around the forearm, one third distal from the elbow joint, similar to previous studies [11 (link),12 (link)]. An example of the placement of the electrodes are shown in Figure 3 a and b. The signals were recorded in monopolar derivation, with the reference electrode on the olecranon, amplified with a gain of 2000, filtered between 10 and 900 Hz, and sampled at 2048 Hz (EMG-USB2, OT Bioelettronica, Italy). All experiments were performed with the arm in neutral position (at the side of the body) and with the elbow close to full extension. The data were processed in real time, and online feedback was provided to the subject. The data processing period was 100 ms.
4
Gait Biomechanics with Surface EMG
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Prior to the experiment, the skin surface of all participants was shaved, abraded and cleaned with alcohol before placing surface electromyography electrodes (EMG) (Ambu Neuroline 720 01-K/12, Ag/AgCl, inter electrode distance 20 mm, Ambu A/S, Ballerup, Denmark) over the following seven muscles of the dominant lower limb: biceps femoris (BF), vastus medialis (VM), vastus lateralis (VL), medial and lateral head of gastrocnemius (GM and GL), soleus (SOL) and tibialis anterior (TA). The electrodes were mounted in accordance to the SENIAM guidelines [Hermens et al, 2000] .
EMG data were collected using a wireless EMG amplifier (TeleMyo 2400 G2 Telemetry System, Noraxon U.S.A. Inc., Arizona, USA) at a sampling rate of 1500 Hz and with an individual specific gain factor (500-1000). Further, a footswitch (DTS Footswitch, Noraxon U.S.A. Inc., Arizona, USA) was mounted under the right foot, enabling the identification of heel strike during the gait cycle. All data were recorded from October through November in 2016.
EMG data were collected using a wireless EMG amplifier (TeleMyo 2400 G2 Telemetry System, Noraxon U.S.A. Inc., Arizona, USA) at a sampling rate of 1500 Hz and with an individual specific gain factor (500-1000). Further, a footswitch (DTS Footswitch, Noraxon U.S.A. Inc., Arizona, USA) was mounted under the right foot, enabling the identification of heel strike during the gait cycle. All data were recorded from October through November in 2016.
5
Transcranial Magnetic Stimulation and Anodal tDCS
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For the 12 participants receiving TMS, the a-tDCS and sham test days started with surface electromyography (sEMG) electrodes being mounted on rectus femoris (RF) on the right leg. First, the skin was shaved, abraded, and cleaned with alcohol, before two surface electromyography electrodes (Ambu Neuroline 720 01-K/12, Ag/AgCl, inter electrode distance 20 mm, Ambu A/S, Ballerup, Denmark) were mounted parallel to the muscle fiber direction according to the SENIAM guidelines. A reference electrode was mounted between the two recording electrodes. A Magstim stimulator (Magstim 200, figure of eight coil shape, coil size 70mm, Magstim Company, Dyfed, UK) was used to determine the hotspot, resting motor threshold (RMT), and MEP amplitude of the RF. To determine the hotspot, three consecutive stimuli over Cz were delivered where after the coil was moved in ~1cm steps laterally, until the highest and most consistent MEPs were elicited. This position was marked on the scalp with a felt pen. Subsequently, RMT was identified, and defined as the minimal stimulus intensity at which 5 out of 10 consecutive stimuli evoked a MEP with an amplitude of at least 50 μV in the resting muscle Twenty MEPs were then elicited at 120% RMT stimulus intensity. MEP´s were recorded at two time points. Firstly, prior to the a-tDCS protocol and secondly five minutes post the stimulation protocol.
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