U343mg

Human glioblastoma cell line U87MG (WHO grade IV) was purchased from the National Institute for Cancer Research of Genoa (Italy). The human T98G and U343MG cell lines (WHO grade IV) were obtained from the American Type Culture Collection (USA) and CellLines Service GmbH (Germany), respectively. Cell lines were controlled for DNA profiling. U343MG cells were maintained in Minimum Essential Medium Eagle with 2 mM l-glutamine (Sigma-Aldrich, Milan, Italy), 1.5 g/L sodium bicarbonate (Sigma-Aldrich, Milan, Italy), 10% FBS (Sigma-Aldrich, Milan, Italy), 100 U/mL penicillin, 100 mg/mL streptomycin (Sigma-Aldrich, Milan, Italy), 1% non-essential amino acids, and 1.0 mM sodium pyruvate (Sigma-Aldrich, Milan, Italy). U87MG and T98G cells were maintained in an RPMI medium supplemented with 10% FBS, 2 mM l-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin, and 1% non-essential amino acids (NEAA) (Sigma-Aldrich, Milan, Italy). HeLa cells were propagated in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (Euroclone) with 10% foetal bovine serum (FBS) (Euroclone). Cell cultures were maintained on a 6-well cell culture cluster (Corning) at 35 °C with 5% CO₂. Mycoplasma contaminations were excluded by PCR using primers GPO-3 and MGSO.

Human GBM cell lines A172 and U343MG were obtained from the American Type Culture Collection (Manassas, VA, USA). These cells were maintained as an adherent monolayer by serial passage at 37 °C under 5% CO₂ conditions and cultured by RPMI 1640 medium supplemented with 10% FBS and 1% penicillin–streptomycin.