IPG strips and buffers were purchased from GE Healthcare (Buckinghamshire, England). Ammonium bicarbonate and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypsin was purchased from Promega (Madison, WI, USA). HCCA and peptide calibration standards were purchased from Bruker Daltonics (Billerica, MA, USA). SDS-PAGE molecular weight standards were purchased from Bio-Rad (Hercules, CA, USA). Anti-acetylated α -tubulin antibody (clone 6-11B-1) was purchased from Funakoshi (Tokyo, Japan). Alexa 488-conjugated anti-mouse antibody was purchased from Invitrogen (Carlsbad, CA, USA). OsO4, propylene oxide and Quetol 812 were purchased from Nisshin EM Co. (Tokyo, Japan). All the other reagents were purchased from Wako Pure Chemicals (Osaka, Japan) or Nacalai Tesque (Kyoto, Japan).
Propylene oxide
Manufactured by Nisshin EM
Sourced in Japan
Propylene oxide is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a precursor in the production of various industrial and consumer products.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Sds page molecular weight standards, by Bio-Rad (1 mentions)
Trypsin, by Promega (1 mentions)
Quetol 812, by Nisshin EM (1 mentions)
H 7650, by Hitachi (1 mentions)
Ultracut s ultramicrotome, by Leica (1 mentions)
2 protocols using propylene oxide
1
Immunofluorescence and Electron Microscopy
2
Ultrastructural Analysis of Murine Renal Cortex
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The renal cortex tissues from 8-week-old C57BL/6 male mice were cut into small pieces and fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer at 4 °C overnight. The specimens were postfixed in 1% osmium tetroxide for four hours, dehydrated in ascending grades (50%, 70%, 80%, 95% once for 20 min and 99.5% 3 times for 20 min) of ethanol. The specimens were then embedded in propylene oxide (no. 311, Nisshin-EM, Tokyo, Japan), a substituting agent, for 10 min followed by incubation overnight in an equal mixture of propylene oxide and Epok 812 (02-1002, Okenshoji Co., Ltd., Tokyo, Japan), followed by the solidification of embedding resin consisting of EPOK812, DDSA (02-1005, Okenshoji Co., Ltd., Tokyo, Japan) and MNA (02-1006, Okenshoji Co., Ltd., Tokyo, Japan) at a ratio of 8 : 7 : 6 with DMP-30 (02-1009, Okenshoji Co., Ltd., Tokyo, Japan) at 1.5%. Serial ultrathin sections were cut using an ultra-cut S ultramicrotome (Leica, Wetzlar, Germany) with a diamond knife and picked up on nickel grids (Okenshoji Co., Ltd., Tokyo, Japan). The sections were double-stained with uranyl acetate and lead citrate. The sections were observed at 80 kV using H7650 (Hitachi, Tokyo, Japan).
All animal experiment protocols were approved by the Ethical Committee on Animal Experiments of the University of Tokyo (M-P16-125) and were conducted in accordance with institutional guidelines.
All animal experiment protocols were approved by the Ethical Committee on Animal Experiments of the University of Tokyo (M-P16-125) and were conducted in accordance with institutional guidelines.
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