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2 protocols using sodium hydrogen phosphate

1

Simvastatin and Poloxamer Lipid Nanoparticles

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Simvastatin and poloxamer 188 and 407 were a kind gift sample from Sun Pharma, India. Lipid and cosurfactant were obtained from Gattefosse, France ex gratia. Formalin, hydrochloric acid, tris-hydrochloric acid, and methyl paraben were purchased from S.D fine chemicals Ltd., Mumbai, India. Bovine serum albumin, carbopol 934, disodium hydrogen phosphate, sodium hydrogen phosphate, thiobarbituric acid and tri-chloro acetic acid were purchased from central drug house Pvt. Ltd., Delhi, India. Chloroform, polyethylene glycol 400, potassium dihydrogen phosphate, and methanol were purchased from Fisher Scientific Pvt. Ltd., Mumbai, India. Hydrogen peroxide, sodium potassium tartarate, and sodium chloride were purchased from LobaChemie Pvt. Ltd., Mumbai, India. Copper sulphate and sodium hydroxide were purchased from Qualigens Fine Chemicals, Mumbai, India. Diethyl ether was purchased from Sisco Research Laboratories, Mumbai, India. Dialysis membrane (cut off of 12,000–14,000 Da) was purchased from Himedia Laboratories Ltd., Mumbai, India.
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2

Quantitative HPLC Analysis of Enrofloxacin and Ciprofloxacin

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Standards of enrofloxacin and ciprofloxacin of purity of 99.9% and triethylamine (TEA) of purity of 99% were purchased from Alfa Aesar. Acetonitrile (CAN) supra gradient HPLC grade and methanol gradient HPLC grade were purchased from Scharlau Chemie-SA. Sodium hydrogen phosphate (99%), dibasic sodium phosphate (98%), orthophosphoric acid (85%), and hydrochloric acid were purchased from Central Drug house-India.
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