Animals were prepared similarly to MRI studies. Immediately after implantation of the stimulation electrodes, animals were paralyzed, and anesthesia was switched to medetomidine. Using a micromanipulator (MP-285; Sutter Instrument Company), a 16 channel laminar probe (A1x16-10mm-100-177-A16; Neuronexus Technologies) was lowered into the dorsal striatum (~5 mm) through a previously drilled craniotomy (0.2 mm anterior and 3.0 mm lateral to bregma). Extracellular signals recorded via the electrodes were amplified using a 1x gain headstage (model E2a; Plexon Inc.) connected to a 50x preamp (PBX-247; Plexon) and digitalized at 50 kHz. A 250 Hz high-pass filtered was applied on the raw signal and single units were sorted using the Offline Sorter software (Plexon) based on their principal components, peak-valley, and non-linear energy ratio prior to sorting units. Time stamps of the sorted units were exported to MATLAB and post-stimulus time histograms and further statistics were computed using custom procedures. The sample size of neurons were chosen such that between-group comparisons provided a power of 0.95 and significance defined by two-tailed t-tests with α = 0.05.
A1x16 10mm 100 177 a16
Manufactured by NeuroNexus
The A1x16-10mm-100-177-A16 is a 16-channel high-density neural probe designed for electrophysiological recordings. It features 16 recording sites spaced at 100 μm intervals along a 10 mm shank with an overall length of 177 mm.
Automatically generated - may contain errors
3 protocols using a1x16 10mm 100 177 a16
1
Striatal Neuronal Activity Recordings
2
Striatal Neuronal Activity Recordings
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Animals were prepared similarly to MRI studies. Immediately after implantation of the stimulation electrodes, animals were paralyzed, and anesthesia was switched to medetomidine. Using a micromanipulator (MP-285; Sutter Instrument Company), a 16 channel laminar probe (A1x16-10mm-100-177-A16; Neuronexus Technologies) was lowered into the dorsal striatum (~5 mm) through a previously drilled craniotomy (0.2 mm anterior and 3.0 mm lateral to bregma). Extracellular signals recorded via the electrodes were amplified using a 1x gain headstage (model E2a; Plexon Inc.) connected to a 50x preamp (PBX-247; Plexon) and digitalized at 50 kHz. A 250 Hz high-pass filtered was applied on the raw signal and single units were sorted using the Offline Sorter software (Plexon) based on their principal components, peak-valley, and non-linear energy ratio prior to sorting units. Time stamps of the sorted units were exported to MATLAB and post-stimulus time histograms and further statistics were computed using custom procedures. The sample size of neurons were chosen such that between-group comparisons provided a power of 0.95 and significance defined by two-tailed t-tests with α = 0.05.
3
Extracellular Neuronal Recordings with Planar Silicone Electrodes
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Electrophysiological recordings were taken with 16-channel, acute single shank planar silicone electrodes (l = 10 mm, base width = 50 µm) with 177 µm2 recording sites spaced 100 µm apart (A1x16-10mm-100-177-A16; Neuronexus Technologies. AnnArbor, MI, US, http://neuronexus.com/electrode-array/a1x16-10mm-100-177/ ). Data acquisition was performed with RHD2000 Evaluation System. The recorded signals were preamplified with a 16-channel headstage/amplifier board (RHD2132 amplifier board, Intan Technologies, Los Angeles, US) under Faraday-cage then the signals were sent through an interface cable to the interface board (RHD2000 USB interface board, Intan Technologies, Los Angeles, US). All recorded data were sampled at 20 kHz, the broad band signals were filtered with a 1-9000 Hz bandpass filter and a notch filter was also applied to eliminate the 50 Hz electrical noise. All data were analyzed off-line in MATLAB environment with implemented toolboxes (Chronux, http://chronux.org/ ; FMAtoolbox, http://fmatoolbox.sourceforge.net ) and custom written scripts.
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