Mcp 1

Manufactured by MyBioSource

Sourced in United States

MCP-1 is a cytokine that functions as a chemotactic factor for monocytes. It plays a role in the recruitment of monocytes to sites of inflammation and injury.

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Lab products found in correlation

Tnf α, by Santa Cruz Biotechnology (2 mentions) Il 10, by Santa Cruz Biotechnology (2 mentions) Nitrotyrosine, by Cayman Chemical (1 mentions) Connexin 43, by Abcam (1 mentions) Fluorometric assay kit, by Abcam (1 mentions) Pentra 400 machine, by Horiba (1 mentions)

Close spelling variants of this product

Rat TNF-α and MCP-1 Immunoassay kit (2 citations)

4 protocols using mcp 1

Oxidative Stress and Inflammation in Myocardial Tissue

Cited 1 time

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Systemic oxidative stress was assessed by IVC levels of 8-epi-isoprostane (EIA kit) [23 (link)]. Myocardial oxidative stress was assessed by in-situ production of superoxide anion with dihydroethidium (DHE) and by expression of the NADPH-oxidase subunits p47 and p67 (1 : 200; both Santa Cruz), and by nitrotyrosine (1 : 200; Cayman Chemical Company, Ann Arbor, Michigan, USA) [23 (link),24 (link)].
Inflammation was evaluated in myocardial sections by standard immunostaining for MCP-1 (1 : 7500; MyBioSource Inc., San Diego, California, USA) and double staining for pro-inflammatory CD68+/iNOS+ (M1) (Abnova Inc., Walnut, California, USA; catalog#: ab15323, 1 : 100) and reparative CD68+/Arinase-1 (M2) (sc-20150 cat#: HPA004114, 1 : 100) macrophages. In addition, myocardial expression of tumor necrosis factor (TNF)-α (Santa Cruz, 1 : 200), interleukin (IL)-6 (1 : 500; Abcam), and IL-10 (1 : 200; Santa Cruz) was quantified by western blot.

Eirin A., Williams B.J., Ebrahimi B., Zhang X., Crane J.A., Lerman A., Textor S.C, & Lerman L.O. (2014). Mitochondrial targeted peptides attenuate residual myocardial damage after reversal of experimental renovascular hypertension. Journal of hypertension, 32(1), 154-165.

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Evaluating Myocardial Inflammation and Remodeling

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Myocardial inflammation was evaluated in 5-µm sections of the LV fluorescently-double stained with connexin-43 (Abcam; Cat# ab79010) and either MCP-1 (MyBioSource, 1:7500), TNF-α (Santa Cruz, 1:200), (IF)-γ (Santa Cruz 1:200), or IL-10 (Santa Cruz 1:200). Images were semiautomatically quantified using ZEN® in 20 random fields, and expressed as percentage of staining of total surface area. Myocyte cross-sectional area (H&E), myocardial fibrosis (trichrome), and interstitial collagen deposition (Sirius red) was assessed in mid-LV cross-sections.

Eirin A., Zhu X.Y., Ebrahimi B., Krier J.D., Riester S.M., van Wijnen A.J., Lerman A, & Lerman L.O. (2014). Intra-renal delivery of mesenchymal stem cells and endothelial progenitor cells attenuates hypertensive cardiomyopathy in experimental renovascular hypertension. Cell transplantation, 24(10), 2041-2053.

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Serum Angiotensin Peptides and Enzyme Activities

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Serum samples were assayed with a Pentra 400 machine and the corresponding assay kits (Horiba France SAS, Longjumeau, France), as described previously [26 (link),27 (link)]. Serum equilibrium levels of angiotensin peptides (Ang I, Ang II, Ang-[1–7], and Ang-[1–5]) were measured using a liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique (Attoquant Diagnostics, Vienna, Austria), as described previously [32 (link)]. Serum ACE activity (Ang II/Ang I) and serum renin activity (Ang I + Ang II) were calculated from the angiotensin peptide levels. Serum ACE2 activity was measured using a fluorometric assay kit (BioVision, Milpitas, CA, USA). Commercial ELISA kits were used to assay interleukin (IL)-6 (Fine Test, Wuhan, China) and monocyte chemoattractant protein-1 (MCP-1) (MyBiosource, San Diego, CA, USA).

Briand F., Sencio V., Robil C., Heumel S., Deruyter L., Machelart A., Barthelemy J., Bogard G., Hoffmann E., Infanti F., Domenig O., Chabrat A., Richard V., Prévot V., Nogueiras R., Wolowczuk I., Pinet F., Sulpice T, & Trottein F. (2022). Diet-Induced Obesity and NASH Impair Disease Recovery in SARS-CoV-2-Infected Golden Hamsters. Viruses, 14(9), 2067.

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Quantifying Inflammatory Markers by ELISA

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Serum IL-6 and TNF-α levels were measured by ELISA (R&D Systems, Minneapolis MN, USA) as well as s-VCAM-1, s-ICAM-1, and MCP-1 (MyBioSource, San Diego CA, USA).

Stasi A., Fiorentino M., Franzin R., Staffieri F., Carparelli S., Losapio R., Crovace A., Lacitignola L., Cimmarusti M.T., Murgolo F., Stufano M., Cafiero C., Castellano G., Sallustio F., Ferrari C., Ribezzi M., Brienza N., Schirinzi A., Di Serio F., Grasso S., Pontrelli P., Tupin C., Barbaras R., Keyserling-Peyrottes C., Crovace A, & Gesualdo L. (2023). Beneficial effects of recombinant CER-001 high-density lipoprotein infusion in sepsis: results from a bench to bedside translational research project. BMC Medicine, 21, 392.

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