Dako real envision system

Manufactured by Agilent Technologies

Sourced in United States

The Dako REAL EnVision system is a laboratory equipment product designed for immunohistochemistry and in situ hybridization applications. It provides a sensitive detection system that enables the visualization of target proteins or nucleic acid sequences in tissue samples.

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Lab products found in correlation

Surgipath, by Leica (1 mentions) Dako diluent, by Agilent Technologies (1 mentions) Mayer s hematoxylin, by Merck Group (1 mentions) A0452, by Agilent Technologies (1 mentions) Scanscope xt system, by Leica (1 mentions) Phospho tdp 43, by Cosmo Bio (1 mentions)

Spelling variants of this product

Envision System (550 citations) Dako Envision system (132 citations) Dako REAL EnVision Detection System (125 citations) Peroxidase/DAB Dako Real EnVision Detection System (19 citations) Dako REAL EnVision Detection System kit (7 citations) Dako REAL EnVision Detection System Peroxidase/DAB+kit (6 citations) DAKO REAL EnVision Inspection System (4 citations) Dako REAL EnVision Detection System K5007 (4 citations) Envision Dako REAL™ EnVision™ Detection System (2 citations)

4 protocols using dako real envision system

Immunohistochemical Analysis of Discarded IMA

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Discarded human IMA were taken from the operation room and placed immediately at -80°C. The snap frozen tissues were embedded with OCT (Surgipath, #01480, Leica Biosystems, Nussloch, Germany) and equilibrate at -20°C for frozen sectioning to 5 μm. Sections were air dried for 5 min and washed to remove OCT. For immunohistochemical staining, each section was blocked with blocking solution for 1 h and incubated for 15 minutes in 3% H2O2 diluted in methanol, with complete washing of sections between each steps. Specimens of LIMA were stained with primary antibodies: rabbit polyclonal to Axl (Abcam, #ab37861, Cambridge, UK,) and rabbit polyclonal to VEGFR2 (#55B11, Cell Signaling Technology, Danvers, MA) diluted in Dako diluent (Dako, #s3022, Dako Cytomation, Glostrup, Sweden) for 1 h at room temperature followed by detection with the Dako REAL EnVision system (Dako, #K5007, Dako Diagnostics, Dublin, Ireland) and mounted under cover slips.

Lee C.H., Shieh Y.S., Hsiao F.C., Kuo F.C., Lin C.Y., Hsieh C.H, & Hung Y.J. (2014). High glucose induces human endothelial dysfunction through an Axl-dependent mechanism. Cardiovascular Diabetology, 13, 53.

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Histological and Immunohistochemical Analysis of Mouse Colon Tissue

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Paraffin-embedded mouse colon tissues were mounted on slides coated with silane (Dako, Santa Clara, CA) and stained with hematoxylin for histological analysis. Hematoxylin-eosin staining was performed according to standard protocols. For immunohistochemistry, slides were deparaffinized in xylene and rehydrated in a series of graded alcohols, and the antigen was retrieved in 0.01 mM sodium citrate buffer. Slides were treated with 3% of hydrogen peroxide to block endogenous peroxidase. After incubation with specific antibody, the Dako REAL EnVision system was used as detection system according to the manufacturer's protocols (Agilent, Santa Clara, CA). The reaction was evaluated by use of the appropriate substrate/chromogen reagent and Mayer's hematoxylin (Sigma-Aldrich) was used for counterstaining.

Kim J.Y., Kim Y.M., Park J.M., Han Y.M., Lee K.C., Hahm K.B, & Hong S. (2017). Cancer preventive effect of recombinant TRAIL by ablation of oncogenic inflammation in colitis-associated cancer rather than anticancer effect. Oncotarget, 9(2), 1705-1716.

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Immunohistochemical Analysis of Tongue Samples

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Immunostaining of tongue sections for VDR, 24-hydroxylase (CYP24A1) and CD3 was performed using the envision technique, Dako real envision System and Peroxidase/DAB+ (Agilent Technologies, Santa Clara, CA, USA), as described previously [17 (link)]. Formalin fixed paraffin embedded (FFPE) tongue sections were stained with antibodies specific for VDR (Thermo Fisher Scientific; MA1-710; 1:500 for 30 minutes at room temperature), CYP24A1 (MyBioSource, Inc.; MBS178241; 1:1000; 1 hour at 37°C) and CD3 (Dako; A0452; 1:200; 30 mins at room temperature). The FFPE slides were digitized using ScanScope XT system (Aperio Technologies). Two-three fields per tongue were captured for each mouse for quantification of VDR, CYP24A1 staining intensity and CD3+ T counts. Quantification of nuclear VDR, cytoplasmic CYP24A1 and CD3 T cells was performed on captured digital images (20X magnification) using the Image J IHC profiler Macro (NIH Image J, Version 1.51j8). A binarized image was created in NIH Image J and used to quantify the number of stained cells in a given field (CD3+ count/field). Positive and negative controls were used to validate the absence of non-specific binding of all antibodies used.

Verma A., Vincent-Chong V.K., DeJong H., Hershberger P.A, & Seshadri M. (2020). Impact of Dietary Vitamin D on Initiation and Progression of Oral Cancer. The Journal of steroid biochemistry and molecular biology, 199, 105603.

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Postmortem Brain Analysis for Neurodegenerative Diseases

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Autopsy was performed 60h after death. Brain specimens were paraffin-embedded, cut into 5-m-thick sections, deparaffinized, and rehydrated. Following epitope unmasking monoclonal antibodies to αsynuclein and p62 (BD Transduction Laboratories, Oxford, UK); β-amyloid (Signet, BioLegend, San Diego, USA); phospho-tau (Innogenetics, Ghent, Belgium); and phospho-TDP-43 (Cosmo Bio, Tokyo, Japan) were applied, incubated overnight (4°C), and detected using the Dako REAL EnVision System (Agilent Technologies, Santa Clara, USA). Alzheimer's disease neuropathological diagnosis was based on βamyloid plaques distribution (according to Thal's phase), neurofibrillary tangle pathology distribution (according to Braak's staging), and neocortical neuritic plaque density (according to CERAD). 6

Straniero L., Guella I., Cilia R., Parkkinen L., Rimoldi V., Young A., Asselta R., Soldà G., Sossi V., Stoessl A.J., Priori A., Nishioka K., Hattori N., Follett J., Rajput A., Blau N., Pezzoli G., Farrer M.J., Goldwurm S., Rajput A.H, & Duga S. (2017). DNAJC12 and dopa-responsive nonprogressive parkinsonism. Annals of neurology, 82(4).

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