Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in Surface Plasmon Resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
Superdex 10 300 gl columns for akta fplc
Manufactured by GE Healthcare
The Superdex 10/300 GL columns are a size exclusion chromatography (SEC) product designed for use with the ÄKTA FPLC (Fast Protein Liquid Chromatography) system. These columns are intended for the separation and purification of biomolecules, such as proteins, peptides, and other macromolecules, based on their size and molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Protein assay kit, by Bio-Rad (2 mentions)
Plasmid miniprep kit, by Qiagen (2 mentions)
Molecular biology grade reagents and chemicals, by Merck Group (2 mentions)
Thrombin cleavage capture kit, by Merck Group (2 mentions)
Amicon spin concentrator, by Merck Group (2 mentions)
Ni2 nta chromatography resin, by Merck Group (2 mentions)
Protein markers for sds page, by Bio-Rad (2 mentions)
Biacore sensor chip nta, by GE Healthcare (2 mentions)
2 protocols using superdex 10 300 gl columns for akta fplc
1
Protein Purification and Characterization
2
Protein Purification Using Ni2+ Chromatography
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Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in surface plasmon resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
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