Serpine2 8c4

Cells were lysed in AB lysis buffer and Western blot analysis was performed. Blots were visualized and bands quantified on an Odyssey imaging system (LI-COR Biosciences, Lincoln, NE, USA) as described(61 (link)). Primary antibodies used were ZEB1 (H-102, Santa Cruz, used at a 1:200 dilution), SEC23A (NBP1-32773, Novus Biologicals, Littleton, CO, USA, used at a 1:5000 dilution), CDH1 (36/E-cadherin, BD Biosciences, used at a 1:5000 dilution), FN1 (C-20, Santa Cruz Biotechnology, Santa Cruz, CA, USA, used at a 1:100 or 1:200 dilution), SERPINE2 (8C4.1, Millipore, Temecula, CA, USA, used at 1:1,000), LRP1 (EPR3724, Abcam, used at 1:5,000), GAPDH (6C5, Thermo Fisher, used at 1:10,000) and ACTB (AC-15, Sigma-Aldrich, St. Louis, MO, USA, used at a 1:10,000 dilution). Secondary antibodies were Anti-Rabbit (Alexa Fluor 680), Anti-Rabbit (Alexa Fluor 800), Anti-Goat (Alexa Fluor 680), Anti-Mouse (Alexa Fluor 680), or Anti-Mouse (Alexa Fluor 800) (Molecular Probes, Eugene, OR, USA), used at a 1:10,000 dilution.