Fn14 knockout (KO) mice [11 (link)] (kindly provided by Biogen, Cambridge, MA, USA) and their inbred wildtype littermates (colonies maintained and bred at the Animal Resources Centre, Murdoch, WA, Australia) were housed on wheaten chaff bedding and kept on 12-h day/night cycles in individually ventilated cages. At six weeks of age, randomly grouped mice were given ad libitum access to either a normal chow and drinking water or a choline-deficient diet (MP Biomedicals, Seven Hills, NSW, Australia) and water that contained 0.15% DL-ethionine (Sigma-Aldrich, Castle Hill, NSW, Australia), as previously described [12 (link)]. Pancreas tissues were harvested three days, three months and six months of CDE treatment. All animal experiments were performed in accordance with the Australian code for the care and use of animals for scientific purposes at Curtin University, Perth, Australia, with local animal ethics committee approval (AEC_2014_28).
Dl ethionine
Manufactured by Merck Group
Sourced in Australia
DL-ethionine is a laboratory reagent used in biochemical research. It is a synthetic amino acid that functions as a methionine antagonist. The product is available for purchase through authorized Merck Group distributors.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulphoxide dmso, by Merck Group (1 mentions)
Gw9662, by Selleck Chemicals (1 mentions)
C57bl 6 mice, by Shanghai Laboratory Animal Center (1 mentions)
Cerulein, by Merck Group (1 mentions)
Wsx 1 mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
Dmxaa, by MedChemExpress (1 mentions)
Choline deficient diet, by MP Biomedicals (1 mentions)
8 protocols using dl ethionine
1
Choline-Deficient Diet Induces Pancreatic Injury in Fn14 KO Mice
2
Modeling Liver Injury in Mice
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C57BL/6 mice were purchased from the Shanghai Laboratory Animal Center of the Chinese Academy of Sciences. 129Sv/Ev wild-type (WT) mice were obtained from the Shanghai Xipuer-Bikai Laboratory Animal Limited Company. Bex1-deficient (Bex1−/−) mice were kindly provided by Professor Frank L. Margolis, University of Maryland, Baltimore, MD, USA [21 (link)]. Female mice 6–8 weeks old were administered a CDE diet (TROPHIC, Nantong, China) supplemented with 0.15% (w/v) d ,l -ethionine (Sigma-Aldrich, St. Louis, MO, USA) in the drinking water for 3 weeks [23 (link)], while control mice received normal chow and drinking water. Rosiglitazone (TCI, Tokyo, Japan) or GW9662 (dose of 2 mg/kg; Selleckchem, Houston, TX, USA) was administered to mice via intraperitoneal injection every second day, for a dose of 50 mg/kg, and dimethyl sulphoxide (DMSO; Sigma-Aldrich) was injected into control mice. All mice were maintained under specific pathogen-free conditions in the vivarium of Shanghai Jiao Tong University School of Medicine. All animal procedures were approved by the Animal Welfare & Ethics Committee of Shanghai Jiao Tong University School of Medicine.
3
Murine models of acute pancreatitis
4
Choline-Deficient Diet-Induced Liver Injury
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We used 6‐8‐week‐old male mice on a C57BL/6 background in this study. IL‐17−/− mice were generously provided by Professor Yoichiro Iwakura (Japan). WSX‐1−/− mice were purchased from the Jackson Laboratory. Mice were fed a control (choline‐sufficient) or choline‐deficient diet (MP Biomedicals, Illkirch, France), and drinking water was supplemented with DL‐ethionine (0.15% weight/volume) (Sigma‐Aldrich, Lyon, France) in the CDE‐fed group. Animals were killed at indicated time points, blood was collected for serum extraction, and the liver was either fixed in buffered formalin or snap frozen in liquid nitrogen. Experiments were performed on at least four animals per group and per time point. All animals were housed and fed ad libitum in a pathogen‐free animal facility and used in accordance with protocols approved by the French ethical committee (COMETH, Authorization N°12‐079) and under the supervision of authorized investigators.
5
Choline-Deficient and DDC Diet Mouse Models
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C57BL/6 mice were purchased from Charles River Laboratories (Wilmington, MA). Mice used in this study were aged 6 weeks and weighed around 19 g. For the CDE diet, mice were given unlimited access to choline-deficient diet (TROPHIC, Nantong, China) and drinking water supplement with 0.15% (w/v) DL-ethionine (Sigma-Aldrich, St. Louis, MO) for 3 weeks. For the DDC diet, 6-week-old male mice were fed a diet supplemented with 0.1% DDC (TROPHIC) for 3 weeks, while control mice received normal chow and drinking water for the entire experimental period and were killed at the same time as mice fed with the CDE and DDC diet. All animal studies were approved by the Committee on the Ethics of animal experiments of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine. All animal experiments in the present study were performed under protocols following the Guide for the Care and Use of Laboratory Animals.
6
Choline-Deficient Diet Induces Liver Injury
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Mice were fed choline-deficient diet (Altromin) and 0.15% DL-ethionine in the drinking water (Sigma-Aldrich) for 3 weeks.
7
Cerulein and CDE Murine Pancreatitis Models
8
Choline-Deficient Ethionine-Supplemented Diet in Mice
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Six-week-old male C57BL/6J mice (Animal Resources Centre, Murdoch, Australia) were housed on wheaten chaff bedding and kept on 12-hour day/night cycles in individually ventilated cages. Randomly grouped mice were exposed to experimental conditions with free access to either a choline-deficient diet (MP Biomedicals, NSW, Australia) with drinking water that contained 0.15% DL-ethionine (Sigma-Aldrich, Castle Hill, NSW, Australia) as previously described (CDE diet 23 ) or normal chow with water that contained 300 mg/L of TAA (Sigma-Aldrich). 12 Control animals received normal chow and drinking water. Liver tissue and serum were harvested on days 3, 7, 14, 21, and 42. All animal experiments were performed in accordance with the Australian code for the care and use of animals for scientific purposes at Curtin University, Perth, Australia, with local animal ethics committee approval.
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