The expression of SEMA3D and PDE1A was assessed by IHC using formalin-fixed paraffin-embedded (FFPE) tissue. The staining antibodies were as follows: SEMA3D (dilution 1/50; Cat.# NBP1-85517, NOVUS, Centennial, United States of America) and PDE1A (dilution 1/200; Cat.# 12442-2-AP, Proteintech, Wuhan, China) (Supplementary Table S3 ). Antibody detection and visualization were performed using DAB (3,3′-diaminobenzidine) as the chromogenic substrate. The images were captured under BA400 Digital microscope (Motic, China). The percentage of DAB-positive tissue in each image was calculated using the Halo data analysis system (Halo 101-WL-HALO-1, Indica labs, United States of America).
Ba400 digital microscope
Manufactured by Motic
Sourced in China
The BA400 Digital Microscope is a high-performance optical instrument designed for various laboratory applications. It features a digital camera and software for image capture and analysis. The microscope utilizes LED illumination and offers a range of magnification options to suit different observation needs.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ba400 digital microscope
1
Immunohistochemistry for SEMA3D and PDE1A
2
Histological and Biochemical Evaluation of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh liver tissues were fixed in 10% neutral formalin, embedded in paraffin, and cut into 5 µm sections. Sections of formalin-fixed livers were used for H&E, Oil red O staining and Sirius Red staining. The stained slices were observed using BA400 Digital microscope (Motic China Group Co., Ltd). The percent of fibrosis areas were calculated by NanoZoomer Digital Pathology S210. Histological steatosis, inflammation, and ballooning were graded in a blinded manner by an experienced pathologist according to the scoring method described by Kleiner et al. (2005) (link). The NAS scores of steatosis, inflammation and ballooning scores were evaluated.
Blood samples obtained from eyelids were used for evaluating the concentration of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) by a chemistry analyzer (Hitachi 7020, Tokyo, Japan) according to the manufacturer’s instructions.
Blood samples obtained from eyelids were used for evaluating the concentration of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) by a chemistry analyzer (Hitachi 7020, Tokyo, Japan) according to the manufacturer’s instructions.
3
Pulmonary Histopathology and Ultrastructure Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the mPAP and RVHI measurements, the rats were sacrificed and the left lung tissues were fixed in 4% paraformaldehyde solution for 48 hours. The tissues were dehydrated and embedded in paraffin. Sections of 5 μm thick were prepared and stained with hematoxylin and eosin (HE) for histopathological evaluation. Morphological changes were observed with a BA400 Digital microscope (Motic China Group Co., Ltd., Chengdu, China) and measured by Image-Pro Plus 6.0 software (Media Cybernetics, Rockville, Maryland, USA). Ultrastructural changes in small pulmonary arteries were examined by means of transmission electron microscopy (TEM). The samples were immersion-fixed with 3% buffered glutaraldehyde. After fixation, the tissues were stored in glutaraldehyde in a refrigerator overnight. The tissue blocks were rinsed in 0.1 M phosphate buffer and post-fixed for 2 h with 1% osmium tetroxide in 0.125 M sodium cacodylate buffer. They were then dehydrated in increasing concentrations (30-100%) of ethanol, rinsed in acetone, and embedded in Araldite. Ultrathin-sections (50 nm) were cut and stained with uranyl acetate and lead citrate and examined with a Hitachi H-600 IV electron microscope (Hitachi High Technologies Co., Ltd., Shanghai, China).
4
Histological Analysis of Rat Synovial Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synovial tissue of the joint from rat was fixed in 4% paraformaldehyde for 24 h. The tissues were dehydrated with ethanol and xylene, fixed in paraffin and sectioned (4 μm) for staining. The synovial tissue of the joint sections was stained with hematoxylin and eosin (H&E) and the images were acquired under a light microscope equipped with 10× or 40× objective lens. For IHC staining, the paraffin sections were deparaffinized with xylene and 3% hydrogen peroxide for antigen retrieval at room temperature for 10 min. Then, the sections were incubated with primary antibodies against RAF(1:500; ab125212; Abcam, CA, USA) at 4 °C overnight, followed by incubation with the appropriate amount of HRP goat anti-rabbit IgG (1:5000; ab205718; Abcam, CA, USA) for 30 min at 37 °C. Next, the reaction was visualized using DAB (Boster, Wuhan, China). Five visual fields were randomly selected and assessed for immunoreactive areas at ×200 magnification using BA400Digital microscope (Motic, Xiamen, China). The optical density of image was analysed by Image-Pro Plus software (Media Cybernetics, Inc., Rockville, MD, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!