Lactose

Manufactured by BD

Sourced in United Kingdom

Lactose is a disaccharide composed of glucose and galactose. It is commonly used in pharmaceutical and biomedical applications as a component in various laboratory equipment and procedures.

Automatically generated - may contain errors

Lab products found in correlation

Kh2po4, by Avantor (2 mentions) Lactose peptone, by BD (2 mentions) Gaspak ez container system, by BD (1 mentions) M17 agar, by BD (1 mentions) Na2hpo4.2h2o, by Avantor (1 mentions) Bacto peptone, by Thermo Fisher Scientific (1 mentions) Sodium metabisulphite, by Merck Group (1 mentions) Corn starch, by Merck Group (1 mentions) Congo red, by BD (1 mentions) Polyvinylpyrrolidone k30, by Merck Group (1 mentions) Sodium bicarbonate, by Loba Chemie (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Citric acid, by Merck Group (1 mentions) Magnesium stearate, by Merck Group (1 mentions)

4 protocols using lactose

Enumeration of Probiotic Bacteria in Yogurt

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On each sampling day, 1 ml of the yogurt sample was serial diluted (1:10) with 0.1% sterile peptone water (Becton Dickinson and Company (BD), Sparks, MD). The aliquots of 100 µL of the diluted samples were plated out onto Difco De Man, Rogosa, and Sharpe (MRS) agar (BD, Sparks, MD) with pH adjusted to 5.2 and M17 agar (BD, Sparks, MD) with 10% w/v lactose (BD, Sparks, MD) to enumerate Lactobacillus delbrueckii subsp. Bulgaricus and Streptococcus thermophiles, respectively. The MRS plates were anaerobically incubated for 72 h at 37 °C using GasPak™ EZ Container Systems (BD, Sparks, MD), and the M17 plates were incubated at 42 °C for 72 h. The counts were presented as logarithms of colony-forming units per mL of yogurt (log CFU/mL). The analyses were carried out in duplicate.

Sarker A, & Ali Siddiqui R. (2023). Effects of ultrasonic processing on the quality properties of fortified yogurt. Ultrasonics Sonochemistry, 98, 106533.

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Cultivation and Development of Dictyostelid Amoebae

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P. pallidum strain PN500_J (
Ppal) was grown in association with
Klebsiella aerogenes at 22°C on 0.1% Lactose-peptone (LP) (1 g Lactose (BDH, UK), 1 g Bacto
^TM Peptone (Gibco; Thermo Fisher Scientific, Inc.), 2.2 g KH
₂PO
₄ (VWR), 1.25 g Na
₂HPO
₄·2H
₂O (VWR) and 15 g agar in 1 L H
₂O) or 1/5
^th SM (Formedium) agar plates. PN500_J is an isolate of
Ppal PN500 with more robust multicellular development. For multicellular development,
Ppal cells were harvested in KK2 (20 mM K-phosphate, pH 6.2) and distributed on non-nutrient (NN) agar (1.5% agar in 8.8 mM KH
₂PO
₄ and 2.7 mM Na
₂HPO
₄) at 10
⁶ cells/cm
²and incubated at 22°C.
D. discoideum (
Ddis) Ax2 cells were grown in HL5 axenic medium and
Ddis NC4 cells were grown in association with
Klebsiella aerogenes on SM agar (Formedium).

Du Q, & Schaap P. (2022). Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas. Open Research Europe, 2, 104.

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Cultivation and Multicellular Development of Protostelium pallidum

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P. pallidum strain PN500_J (Ppal) was grown in association with Klebsiella aerogenes at 22°C on 0.1% Lactose-peptone (LP) (1 g Lactose (BDH, UK), 1 g Bacto™ Peptone (Gibco; Thermo Fisher Scientific, Inc.), 2.2 g KH₂PO₄ (VWR), 1.25 g Na₂HPO₄o2H₂O (VWR) and 15 g agar in 1 L H₂O) or 1/5^th SM (Formedium) agar plates. PN500_J is an isolate of Ppal PN500 with more robust multicellular development. For multicellular development, Ppal cells were harvested in KK2 (20 mM K-phosphate, pH 6.2) and distributed on non-nutrient (NN) agar (1.5% agar in 8.8 mM KH₂PO₄ and 2.7 mM Na₂HPO₄) at 10⁶ cells/cm² and incubated at 22°C. D. discoideum (Ddis) Ax2 cells were grown in HL5 axenic medium and Ddis NC4 cells were grown in association with Klebsiella aerogenes on SM agar (Formedium).

Du Q, & Schaap P. (2022). Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas. Open Research Europe, 2, 104.

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Glipizide Extended-Release Formulation

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Glipizide (gift sample from Beximco Pharma Ltd., Bangladesh), Polyvinylpyrrolidone K30 (Merck, Germany), Corn Starch (Merck, India), Sodium bicarbonate (LOBA Chemie, India), Citric acid, Ascorbic acid (Merck, India), Sodium Saccharine (HCT, China), Lactose (BDH, England), Magnesium stearate (Merck, Germany), Sodium Metabisulphite (Merck, India), Congo red (BDH, England). All the reagents were purchased through RFQ from local Suppliers.

Aklima A., Baral P.K., Amin M.T., Emon T.I., & Hossain M.S. (2020). Formulation and Quality Optimization of Effervescent Tablet of Glipizide: An Approach to Comfort Anti-Diabetic Medication. Modern Health Science, 3(2), p14-p14.

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