Anti jab1 antibody

RPMI-1640 cell basal culture medium was purchased from Invitrogen (Carlsbad, CA, USA), and fetal bovine serum (FBS) was purchased from Gibco (Grand Island, NY, USA). The total RNA extraction kit and Lipofectamine 2000 transfection reagent were also purshased from Invitrogen (Carlsbad, CA, USA). Si-MRPS30-DT and the negative control (si-NC) were purchased from GenePharma (Shanghai, China). The anti-Jab1 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-GAPDH was purchased from Proteintech (Wuhan, China).

Western blot analyses were performed as described previously 15. Primary antibodies used for western blot and dilution are as follows: anti‐FLAG M2–horse radish peroxidase (HRP) antibody (Sigma‐Aldrich, 1 : 1000), anti‐myc–HRP antibody (Thermo Fisher Scientific, 1 : 5000) and anti‐V5–HRP antibody (Thermo Fisher Scientific, 1 : 5000), anti‐Lamin A/C antibody (Cell Signaling Technology, Danvers, MA, USA, 1 : 1000), anti‐glyceraldehyde‐3‐phosphate dehydrogenase (Cell Signaling Technology, 1 : 500), anti‐p27^kip1 antibody (MBL, 1 : 1000), anti‐JAB1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA, 1 : 200), anti‐Cdk2 antibody (Santa Cruz Biotechnology, 1 : 200), and anti‐cyclin E antibody (MBL, 1 : 1000). Anti‐mouse IgG HRP‐linked and anti‐rabbit IgG HRP‐linked (Cell Signaling Technology, 1 : 5000) secondary antibodies were used. Data were analyzed using lumivision analyzer 400 software (Aisin Seiki, Kariya, Aichi, Japan).