The resuspended BALF cells were used for immune-inflammatory cell counts with cell-type-specific labelled monoclonal antibodies. Total 1.0 × 105 BALF cells were used for antibody labeling. Before antibody staining, all cells were blocked with purified anti-mouse CD16/32 (Cat# 50-163-432, Fisher Scientific) to prevent non-specific binding, and washed with PBS once. The cells were stained with F4/80 PE-conjugated antibody for macrophages (Cat# 123109, BioLegend), LY6B.2 Alexa fluor488-conjugated antibody for neutrophils (Cat# NBP213077AF488, Novus Biologicals), PE-Cyanine7 antibody for CD4a+ T-lymphocytes (Cat# 25-0041-82, Fisher Scientific), and APC conjugated Monoclonal Antibody for CD8a+ T-Lymphocytes (Cat# 17-0081-82, Fisher Scientific). The absolute cell numbers of macrophages, neutrophils, and CD4a+/CD8a+ T-lymphocytes were determined by multiplying the percentage of cells by the total cell counts. Flow cytometry was performed using the Guava® easyCyte™ flow cytometer (Millipore Sigma) and analyzed using Guava® InCyte™ software.
F4 80 pe conjugated antibody for macrophages
Manufactured by BioLegend
The F4/80 PE-conjugated antibody is a laboratory reagent used to identify and analyze macrophages in various biological samples. It binds specifically to the F4/80 antigen, a glycoprotein expressed on the surface of macrophages. This antibody is conjugated with the fluorescent dye Phycoerythrin (PE), allowing for the detection and quantification of macrophages in flow cytometry or other fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Guava easycyte flow cytometer, by Merck Group (2 mentions)
Anti mouse cd16 32, by Thermo Fisher Scientific (2 mentions)
Ly6b 2 alexa fluor488 conjugated antibody for neutrophils, by Novus Biologicals (2 mentions)
Pe cyanine7 antibody for cd4a t lymphocytes, by Thermo Fisher Scientific (2 mentions)
Apc conjugated monoclonal antibody for cd8a t lymphocytes, by Thermo Fisher Scientific (2 mentions)
Accuri flow cytometer, by BD (2 mentions)
Diff quik, by Siemens (1 mentions)
Cd8a pe cy5 conjugated antibody for t, by BD (1 mentions)
4 protocols using f4 80 pe conjugated antibody for macrophages
1
Quantification of BALF Immune Cells
2
Quantifying Immune Cells in BALF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The resuspended BALF cells were used for immune-inflammatory cell counts with cell-type-specific labelled monoclonal antibodies. Total 1.0 × 105 BALF cells were used for antibody labeling. Before antibody staining, all cells were blocked with purified anti-mouse CD16/32 (Cat# 50–163-432, Fisher Scientific) to prevent non-specific binding, and washed with PBS once. The cells were stained with F4/80 PE-conjugated antibody for macrophages (Cat# 123109, BioLegend), LY6B.2 Alexa fluor488-conjugated antibody for neutrophils (Cat# NBP213077AF488, Novus Biologicals), PE-Cyanine7 antibody for CD4a+ T-lymphocytes (Cat# 25–0041-82, Fisher Scientific), and APC conjugated Monoclonal Antibody for CD8a+ T-Lymphocytes (Cat# 17–0081-82, Fisher Scientific). The absolute cell numbers of macrophages, neutrophils, and CD4a+/CD8a+ T-lymphocytes were determined by multiplying the percentage of cells by the total cell counts. Flow cytometry was performed using the Guava® easyCyte™ flow cytometer (Millipore Sigma) and analyzed using Guava® InCyte™ software.
3
Differential Cell Counts and Flow Cytometry in Exposed Mice
4
Inflammatory Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell-type specific monoclonal antibodies were used for flow cytometric analysis of inflammatory immune cells. Briefly, 2.0 × 105 to 4.0 × 105 cells were stained with 1x PBS using cell-type specific markers for 30 min., then washed and re-suspended in 0.1 ml of 1x PBS for analysis. Cell-specific markers such as LY6B.2 Alexa Fluor 488-conjugated antibody for neutrophils (Novus Biologicals Cat# NBP213077AF488) CD8a PE-cy5-conjugated antibody for T-lymphocytes (BD Biosciences 553034) and F4/80 PE-conjugated antibody for macrophages (BioLegend Cat #123109) were used. Flow cytometry data acquisition was performed on a BD Accuri flow cytometer (BD Accuri C6 software) and analyzed using FlowJo software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!