Rabbit anti s1 antibody

DVS, OVS, and MTE mRNA were transfected into 293T cells with MessengerMax (Invitrogen). After 48h, we evaluated the expression of the three mRNAs with flow cytometry (FC), immunofluorescence (IF), and western blot (WB). For IF, cells were incubated with rabbit anti-s1 antibody (Sino Biological) and AF488 conjugated anti-Rabbit secondary antibody (Abcam), and images were taken with the machine. For FC, cells were trypsinized and incubated with rabbit anti-s1antibody (Sino Biological) and AF488 conjugated anti-Rabbit secondary antibody (Abcam). Data was acquired with C6 (BD Biosciences) and analyzed with FlowJo (BD Biosciences). For WB, cells were harvested and denatured in lysis buffer. Samples were loaded and run in 4-12% gradient SDS-PAGE gel and transferred to the PVDF membrane. PVDF membrane was incubated with mouse anti-s2 monoclonal antibody (Thermofisher, Cat# MA5-35946) and HRP conjugated anti-mouse secondary antibody (Invitrogen, Cat#62-6520). Anti-beta-Actin HRP Antibody for protein loading control was purchased from Santa Cruz Biotechnology (sc-47778 HRP).