M7 sphygmomanometer hem 780 e

All experiments were performed in accordance with relevant guidelines and regulations. Systolic blood pressure (SBP) and diastolic BP (DBP) were measured three times, with intervals of 5 minutes, in a seating position from the right arm using a standard Omron M7 sphygmomanometer (HEM-780-E) and the mean value was considered as the subject’s SBP and DBP. All measurements of lipid concentrations were assessed in venous samples drawn after 12–14 h overnight fasting. According to the standard protocol, a 10 ml sample of venous blood, collected in 4 tubes, was drawn from each individual by trained laboratory staff and was sent to collaborating centers. Samples were immediately centrifuged (1500 rpm for 10 min) at each designated laboratory, and transferred under cold chain conditions to the Central Reference Laboratory of Ministry of Health of Iran (Tehran, Iran). Serum lipids including total cholesterol, triglycerides, LDL-C and high density lipoprotein (HDL-C) were determined by enzymatic methods (Parsazmun, Karaj, Iran). Enzymatic calorimetric methods with a glucose oxidize test was used for measuring fasting plasma glucose (FPG). Inter- and intra-assay coefficients of variation (CV) at baseline and follow-up phases were 2.1% and 2.6%, respectively^{33 (link)}.

After at least 10 min of resting in the sitting position two measurements of blood pressure (BP) were obtained within 5 minutes of each other, from the right arm of participants; by trained health-staff using an appropriate sized cuff [20 (link)]. Blood pressure was measured manually with a calibrated Omron M7 sphygmomanometer (HEM-780-E), and the mean of the two abovementioned measurements was considered as the participant’s BP.
BMI was calculated as the weight in kilograms divided by the square of height in meters.
Physical activity was obtained using the global physical activity questionnaire (GPAQ), a standardized questionnaire developed by WHO, as a physical activity surveillance instrument. The translation of the second version of GPAQ was used for the assessment of physical activity [21 (link)]. Another questionnaire was used to collect demographic data, including occupation, sex, age, province, and area.