Kryptofix 222 k2.2.2

Manufactured by Merck Group

Sourced in United States

Kryptofix 222 (K2.2.2) is a macrocyclic polyether compound used in various laboratory applications. It functions as a complexing agent, capable of encapsulating and stabilizing certain metal ions. The compound's chemical structure and properties make it a useful tool in various analytical and synthetic procedures.

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Lab products found in correlation

Oasis hlb cartridge, by Waters Corporation (1 mentions) Acetonitrile mecn, by Merck Group (1 mentions) Sep pak plus c18 cartridge, by Waters Corporation (1 mentions) Ethyl 2 bromopropionate, by Merck Group (1 mentions) Anhydrous dimethylformamide, by Thermo Fisher Scientific (1 mentions) Lc 2010a ht system, by Bioscan (1 mentions) Silica gel 60 tlc plate, by Merck Group (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Anhydrous acetonitrile, by Merck Group (1 mentions) B fc 1000, by Bioscan (1 mentions)

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Kryptofix® 222 (11 citations)

2 protocols using kryptofix 222 k2.2.2

Radiolabeling of [18F]-FDG Tracer

Cited 1 time

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All chemicals obtained commercially were used without further purification unless otherwise indicated. [¹⁸F]-FDG was prepared as previously reported [28 (link)]. Ethyl-2-bromopropionate, acetonitrile (MeCN), dimethylsulfoxide (DMSO), N,N-diisopropylethylamine (DIPEA), bis(4-nitrophenyl)carbonate (NPC), tri-fluoroacetic acid (TFA), and Kryptofix 222 (K2.2.2) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Sep-Pak light QMA cartridges, Sep-Pak plus C18 cartridges, and Oasis HLB cartridges were achieved from Waters Corporation (Milford, MA, USA). Sep-Pak light QMA cartridges were preconditioned with 5 ml NaHCO₃ aqueous (8.4%) and 10 ml H₂O before using. Sep-Pak plus C18 and Oasis HLB cartridges were preconditioned with 10 ml ethanol and water, respectively, before using. High-performance liquid chromatography (HPLC) separation was performed on at the PET-MF-2V-IT-1 synthesizer (PET Co. Ltd., Beijing, China) building HPLC system with a semipreparative reverse-phase C18 column (106 and 250 mm) equipped with a UV detector (Alltech 201, USA) and a radioactivity detector (PET Co. Ltd., China). The mobile phase was 50% solvent A (0.1% TFA in water): 50% solvent B (0.1% TFA in MeCN) with the flow rate of 4 ml/min.

Zhao J., Wang Y.L., Li X.B., Gao S.Y., Liu S.Y., Song Y.K., Wang J.Y., Xiong Y., Ma H., Jiang L., Yang Z.Y., Tang G.H, & Chu J.P. (2018). Radiosynthesis and Preliminary Biological Evaluation of 18F-Fluoropropionyl-Chlorotoxin as a Potential PET Tracer for Glioma Imaging. Contrast Media & Molecular Imaging, 2018, 8439162.

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Radiosynthesis of [18F]Tetramethylammonium Fluoride

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HPLC grade acetonitrile, potassium trifluoromethanesulfonate, and anhydrous dimethylformamide were purchased from Fisher Scientific. Sodium bicarbonate, kryptofix^® 2.2.2 (K_2.2.2), and anhydrous acetonitrile were purchased from Sigma-Aldrich. Tetramethylammonium bicarbonate (Me₄NHCO₃) was purchased from Enamine. Tetramethylammonium tetrafluoroborate (Me₄NBF₄) was purchased from TCI America. Sterile product vials (10 mL) were purchased from Hollister-Stier. QMA-light and C18 Lite Sep-Paks were purchased from Waters Corporation. QMA-light Sep-Paks were flushed with isopropanol (10 mL), 0.5 M aqueous Sodium bicarbonate (10 mL), and MilliQ water (10 mL) prior to use for the generation of K¹⁸F and in situ Me₄N¹⁸F. C18 Sep-Paks were flushed with ethanol (10 mL) and sterile water for injection (10 mL) prior to use. High performance liquid chromatography (HPLC) was performed using a Shimadzu LC-2010A HT system equipped with a Bioscan B-FC-1000 radiation detector. Radio-TLC analyses were performed using a Bioscan AR 2000 Radio-TLC scanner with EMD Millipore TLC silica gel 60 plates (3.0 cm wide x 6.5 cm long). Residual TMA levels were determined using TLC spot tests as previously described.^{32 (link)} Chromatography was performed using the following conditions:

Lee S.J., Morales-Colón M.T., Brooks A.F., Wright J.S., Makaravage K.J., Scott P.J, & Sanford M.S. (2021). SNAr Radiofluorination with In Situ-Generated [18F]Tetramethylammonium Fluoride. The Journal of organic chemistry, 86(20), 14121-14130.

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