Spinal cord sections were processed as above. For immunofluorescence, slides were first desiccated for at least two hours and blocked with 5% Normal Donkey Serum with or without 0.3% Triton-X 100. Primary antibodies were incubated overnight at 4°C: goat anti-CXCL1 1:50 (R&D Systems, Minneapolis, MN), rabbit anti – GFAP 1:500 (Life Technologies, Carlsbad, CA), rat anti – Ly-6B.2 1:100 (Serotec, Raleigh, NC), rabbit anti - Iba11:500 (Wako Chemicals, Richmond, VA), rabbit anti – GST-π 1:1000 (MBL, Woburn, MA).
Goat anti cxcl1
Manufactured by R&D Systems
Goat anti-CXCL1 is a polyclonal antibody produced in goats that specifically recognizes the CXCL1 protein. CXCL1 is a chemokine that plays a role in the inflammatory response.
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Lab products found in correlation
3 protocols using goat anti cxcl1
1
Spinal Cord Immunofluorescence Staining
2
Quantification of Inflammatory Cells in Spinal Cord
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Spinal cord sections were processed as described above. For immunofluorescence, slides were first desiccated for two hours and blocked with 5% Normal Donkey Serum with or without 0.3% Triton‐X 100. Primary antibodies were incubated overnight at 4°C: goat anti‐CXCL1 1:50 (R&D Systems, Minneapolis, MN), rabbit anti‐GFAP 1:500 (Life Technologies, Carlsbad, CA), and rat anti‐Ly6B.2 1:100 (Serotec, Raleigh, NC). Images were analyzed using the Image J software (NIH) according to previously described methods 61 , 62 . Quantification of Ly6B.2‐positive cells within spinal cords of experimental mice was determined by counting cells in a minimum of five spinal cord sections/per mouse with a minimum of three mice per group.
3
Spinal Cord Immunofluorescence Analysis
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