Advanced 3320 osmometer

Manufactured by Advanced Instruments

Sourced in Australia

The Advanced 3320 osmometer is a laboratory instrument designed to measure the osmolality of solutions. It uses freezing point depression to determine the concentration of dissolved solutes in a liquid sample.

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Lab products found in correlation

Chemiluminescent analyzer, by DiaSorin (2 mentions) Advia 2400, by Siemens (2 mentions) Advia 2400 chemistry system, by Siemens (1 mentions) Liaison aldosterone kit, by DiaSorin (1 mentions) Aldosterone elisa kit, by Abcam (1 mentions) Spark 10m, by Tecan (1 mentions)

Spelling variants of this product

Advanced® Model 3320 Micro-Osmometer (7 citations) Osmometer 3320 (5 citations)

3 protocols using advanced 3320 osmometer

Electrolyte and Aldosterone Measurement

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Electrolytes and aldosterone levels in blood and urine were carried out by SA Pathology. Urinary and plasma Na⁺, K⁺, Cl⁻ and creatinine were measured using an Advia 2400 Chemistry System (Siemens, Bayswater, VIC, Australia), and urine osmolality was measured with an Advanced 3320 Osmometer (Advanced Instruments, Thermo Fisher Scientific, Waltham, MA, USA). Plasma aldosterone levels were measured using a Liaison Aldosterone Kit and a Chemiluminescent Analyzer (Diasorin, Saluggia, Italy). GFR was estimated based on creatinine clearance using a standard formula: GFR=urine flow x [urine creatinine]/[plasma creatinine].^{37 (link)}

Henshall T.L., Manning J.A., Alfassy O.S., Goel P., Boase N.A., Kawabe H, & Kumar S. (2017). Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death and Differentiation, 24(12), 2150-2160.

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Comprehensive Kidney Function Analysis

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Electrolytes and other parameters of kidney function in blood were carried out by SA Pathology (Adelaide, Australia). Urine osmolality was measured using an Advanced 3320 osmometer (Advanced Instruments) and other parameters using an Advia 2400 chemistry system (Siemens). Serum aldosterone levels were measured using an Aldosterone Elisa Kit (Abcam, ab136933) and analyzed on a Spark 10 M microplate reader (Tecan, Switzerland).

Manning J.A., Shah S.S., Nikolic A., Henshall T.L., Khew-Goodall Y, & Kumar S. (2021). The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease. Cell Death & Disease, 12(4), 398.

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Kidney Function Biomarker Analysis

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Electrolytes, aldosterone, and other parameters of kidney function in blood were measured at SA Pathology. Plasma Na⁺, K⁺, Cl⁻, Ca²⁺, and creatinine were measured using an Advia 2400 chemistry system (Siemens), and urine osmolality with an Advanced 3320 osmometer (Advanced Instruments). Plasma aldosterone levels were measured using a Liaison Aldosterone Kit and a Chemiluminescent Analyzer (Diasorin, Saluggia, Italy).

Manning J.A., Shah S.S., Henshall T.L., Nikolic A., Finnie J, & Kumar S. (2019). Dietary sodium modulates nephropathy in Nedd4-2-deficient mice. Cell Death and Differentiation, 27(6), 1832-1843.

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