Fitc nk1

Nr-CWS was supplied by Greatest Biopharma Limited Company (purity>98%). Fluorescein conjugated anti-mouse antibodies APC-NK1.1, FITC-NK1.1, APC/Cy7-CD3ε, PE/Cy7-CD3ε, APC-CD4, PE-CD8α, PerCP/Cy5.5-CD11b, PE-CD27, FITC-TNF-α, and PerCP/Cy5.5-IFN-γ from BioLegend, and PerCP/Cy5.5-CD69, FITC-FasL, PE-TRAIL, PE-Perforin, and FITC-Granzyme-B from eBioscience were used in this study. TUNEL assay kit and MTS Cell proliferation, cytotoxicity or chemosensitivity assays kit (cat # 3580) were obtained from Promega. The LEGENDplex™ Multiplex mouse cytokine kit was provided by BioLegend. EasySep™ mouse NK cell isolation kit was from STEMCELL Technologies Inc. Recombinant murine IL-2 was from PeproTech Inc.

PE-CD80, PE-CD83 and FITC-CD40 antibodies were purchased from Ebioscience, USA (12-0801, 12-0831 and 11-0402). Lipopolysaccharide (LPS) was purchased from Sigma, USA (L3012). Mouse GM-CSF and IL-4 were from Sino Biological, China (51048-M07H, 51084-M08B). Anti-CD3 and anti-CD28 antibodies were obtained from Ebioscience, USA (16-0031-82, 16-0281-82). Aluminum hydroxide was from Thermo Fisher, USA (77161). Peroxidase-labeled goat anti-mouse IgE, IgG1 and IgG2a Fc antibody were from Southernbiotech, USA (1110-05, 1070-05 and 1155-05). ELISA kits for IL-5 and IL-13 detection were from 4A Biotech, China (CME0003, CME0009). ELISA kits for IL-4 and IFN-γ were purchased from Ebioscience, USA (88-7044, 88-7314). Anti-mouse TLR2 antibody and Mouse IgG1, κ Isotype Ctrl were obtained from Biolegend, USA (121802, 400101). TLR4 signaling inhibitor was from Invivogen, USA (CLI-095). DNase I and collagenase D were from Sangon Biotech, China (B002004 and A004186). APC-CD45, FITC-NK-1.1, FITC-CD19 and PE-CD90 were obtained from Biolegend, USA (103111, 108705, 115505 and 205903). PerCP-IL-33R and FITC-Lineage antibodies were purchased from Ebioscience, USA (46-9333, 22-7770). PerCP-CD4, FITC-IL-4, PE-IFN-γ, PE-CD11c and PerCP-Siglec-F were purchased from Ebioscience, USA (46-0041, 11-7042, 12-7311, 12-0114 and 46-1702).