Genomic DNA was extracted from whole blood with Flexigen kit (QiagenInc, Valencia, California, USA) at the Basque Biobank for Research-OEHUN. DNA concentration was measured using a NanoDrop Spectrophotometer (NanoDrop Technologies, Inc, Wilmington, DE).
For RNA extraction, fresh peripheral blood mononuclear cells (PBMC) were isolated from heparinized venous blood by centrifugation on a Ficoll-Paque PLUS (GE Healthcare Bio-Sciences, Piscataway, NJ, USA). Total RNA from PBMCs was isolated with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) followed by RNeasy Mini Kit (Qiagen, Santa Clara, CA, USA). Purified total RNA was retrotranscribed to cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), according to manufacturer’s instructions.
For RNA extraction, fresh peripheral blood mononuclear cells (PBMC) were isolated from heparinized venous blood by centrifugation on a Ficoll-Paque PLUS (GE Healthcare Bio-Sciences, Piscataway, NJ, USA). Total RNA from PBMCs was isolated with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) followed by RNeasy Mini Kit (Qiagen, Santa Clara, CA, USA). Purified total RNA was retrotranscribed to cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), according to manufacturer’s instructions.